Emergent Leader Cells in Collective Cell Migration in In Vitro Wound Healing Assay

Persistent Link:
http://hdl.handle.net/10150/332896
Title:
Emergent Leader Cells in Collective Cell Migration in In Vitro Wound Healing Assay
Author:
Yang, Yongliang
Issue Date:
2014
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Collective cell migration is critical for various physiological and pathological processes. In vitro wound healing assay has been widely used to study collective cell migration due to its technical simplicity and ability of revealing the complexity of collective cell migration. This project studies the function and importance of leader cells, the cells pulling cell monolayer migrating into free space, in endothelium and skin epithelial regeneration via plasma lithography enhanced in vitro wound healing assay. Despite leader cells have been identified in in vitro wound healing assays, little is known about their regulation and function on collective cell migration. First, I investigated the role of leader cells in endothelial cell collective migration. I found that the leader cell density is positively related with the cell monolayer migration rates. Second, we used this knowledge to study the effects of arsenic treatment on skin regeneration via in vitro wound healing assay. We found that low concentration of arsenic treatment can accelerate the keratinocyte monolayer migration. We further found that arsenic affected cell migration by modulating leader cell density through Nrf2 signaling pathway. As a conclusion of these studies, we evaluated the function of leader cells in collective cell migration, and elucidated the mechanism of arsenic treatment on skin regeneration.
Type:
text; Electronic Dissertation
Keywords:
endothelial cell; leader cell; monolayer migration; skin regeneration; Mechanical Engineering; collective cell migration
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Mechanical Engineering
Degree Grantor:
University of Arizona
Advisor:
Wong, Pak Kin

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleEmergent Leader Cells in Collective Cell Migration in In Vitro Wound Healing Assayen_US
dc.creatorYang, Yongliangen_US
dc.contributor.authorYang, Yongliangen_US
dc.date.issued2014-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractCollective cell migration is critical for various physiological and pathological processes. In vitro wound healing assay has been widely used to study collective cell migration due to its technical simplicity and ability of revealing the complexity of collective cell migration. This project studies the function and importance of leader cells, the cells pulling cell monolayer migrating into free space, in endothelium and skin epithelial regeneration via plasma lithography enhanced in vitro wound healing assay. Despite leader cells have been identified in in vitro wound healing assays, little is known about their regulation and function on collective cell migration. First, I investigated the role of leader cells in endothelial cell collective migration. I found that the leader cell density is positively related with the cell monolayer migration rates. Second, we used this knowledge to study the effects of arsenic treatment on skin regeneration via in vitro wound healing assay. We found that low concentration of arsenic treatment can accelerate the keratinocyte monolayer migration. We further found that arsenic affected cell migration by modulating leader cell density through Nrf2 signaling pathway. As a conclusion of these studies, we evaluated the function of leader cells in collective cell migration, and elucidated the mechanism of arsenic treatment on skin regeneration.en_US
dc.typetexten
dc.typeElectronic Dissertationen
dc.subjectendothelial cellen_US
dc.subjectleader cellen_US
dc.subjectmonolayer migrationen_US
dc.subjectskin regenerationen_US
dc.subjectMechanical Engineeringen_US
dc.subjectcollective cell migrationen_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMechanical Engineeringen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWong, Pak Kinen_US
dc.contributor.committeememberWong, Pak Kinen_US
dc.contributor.committeememberZohar, Yishaken_US
dc.contributor.committeememberWu, Xiaoyien_US
dc.contributor.committeememberLi, Peiwenen_US
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