Mixotrophic Cultivation Of The Microalga Scenedesmus obliquus With Reused Municipal Wastewater

Persistent Link:
http://hdl.handle.net/10150/332836
Title:
Mixotrophic Cultivation Of The Microalga Scenedesmus obliquus With Reused Municipal Wastewater
Author:
Liao, Yang
Issue Date:
2014
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Scenedesmus obliquus is a freshwater microalga which has high lipid content and biomass productivity. It is regarded as a promising species for production of biodiesel and other valuable organic compounds. Given the high cost of using potable water and commercial fertilizers, the use of municipal wastewater as algal growth medium is attractive in view of its constituent organic carbon and inorganic nutrients, including nitrogen and phosphorus. Investigating the mixotrophic cultivation of S. obliquus in an imitation municipal wastewater, the results of this study showed that: (1) The unmodified imitation wastewater by itself as expected yielded poor S. obliquus growth owing to its pH significantly decreasing to 3.5 as caused by the presence of Ammonium Chloride in the wastewater, inhibiting cell growth; (2) Adding either Acetic Acid or Sodium Acetate to the wastewater medium maintained its pH at 6.5 to 7.0, and its algae biomass on day 6 increased significantly by 212% and 194%, respectively; (3) Adding either Acetic Acid or Sodium Acetate to the wastewater medium maintained its pH at 6.5 to 7.0, and its algae biomass during exponential phase (day 4) significantly exceeded that in the MF control by 220.6% and 165.8%, respectively, while its algae biomass during saturation (day 6) significantly exceeded that in the MF control by 60.8% and 51.5%, respectively; and (4) Addition of NaNO₃ to the wastewater to match the level of N in the MF medium improved the algae biomass by 10%. This study developed ways for how the successful mixotrophic cultivation of S. obliquus in municipal wastewater could be achieved.
Type:
text; Electronic Thesis
Keywords:
Scenedesmus obliquus; wastewater medium; Agricultural & Biosystems Engineering; mixotrophic cultivation
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Agricultural & Biosystems Engineering
Degree Grantor:
University of Arizona
Advisor:
Cuello, Joel L.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleMixotrophic Cultivation Of The Microalga Scenedesmus obliquus With Reused Municipal Wastewateren_US
dc.creatorLiao, Yangen_US
dc.contributor.authorLiao, Yangen_US
dc.date.issued2014-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractScenedesmus obliquus is a freshwater microalga which has high lipid content and biomass productivity. It is regarded as a promising species for production of biodiesel and other valuable organic compounds. Given the high cost of using potable water and commercial fertilizers, the use of municipal wastewater as algal growth medium is attractive in view of its constituent organic carbon and inorganic nutrients, including nitrogen and phosphorus. Investigating the mixotrophic cultivation of S. obliquus in an imitation municipal wastewater, the results of this study showed that: (1) The unmodified imitation wastewater by itself as expected yielded poor S. obliquus growth owing to its pH significantly decreasing to 3.5 as caused by the presence of Ammonium Chloride in the wastewater, inhibiting cell growth; (2) Adding either Acetic Acid or Sodium Acetate to the wastewater medium maintained its pH at 6.5 to 7.0, and its algae biomass on day 6 increased significantly by 212% and 194%, respectively; (3) Adding either Acetic Acid or Sodium Acetate to the wastewater medium maintained its pH at 6.5 to 7.0, and its algae biomass during exponential phase (day 4) significantly exceeded that in the MF control by 220.6% and 165.8%, respectively, while its algae biomass during saturation (day 6) significantly exceeded that in the MF control by 60.8% and 51.5%, respectively; and (4) Addition of NaNO₃ to the wastewater to match the level of N in the MF medium improved the algae biomass by 10%. This study developed ways for how the successful mixotrophic cultivation of S. obliquus in municipal wastewater could be achieved.en_US
dc.typetexten
dc.typeElectronic Thesisen
dc.subjectScenedesmus obliquusen_US
dc.subjectwastewater mediumen_US
dc.subjectAgricultural & Biosystems Engineeringen_US
dc.subjectmixotrophic cultivationen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineAgricultural & Biosystems Engineeringen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorCuello, Joel L.en_US
dc.contributor.committeememberCuello, Joel L.en_US
dc.contributor.committeememberSlack, Donald C.en_US
dc.contributor.committeememberAn, Linglingen_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.