Engineered Molecular Probes for Systematic Studies of Cellular Response in Collective Cell Migration

Persistent Link:
http://hdl.handle.net/10150/312515
Title:
Engineered Molecular Probes for Systematic Studies of Cellular Response in Collective Cell Migration
Author:
Riahi, Reza
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The investigation of complex biological processes, such as wound healing, cell migration, cancer cell invasion, and gene regulatory networks can be benefited tremendously by novel biosensing techniques with high stability and spatiotemporal resolution. In particular, molecular probes with qualities including high stability, sensitivity, and specificity are highly sought-after for long-term monitoring of gene expression in individual cells. Among different single-cell analysis techniques oligonucleotide optical probes is a promising detection method to monitor the dynamics of cellular responses. Herein, the design and optimization of double-stranded LNA probes are first investigated. With alternating DNA/LNA monomers for optimizing the stability and specificity, we show that the probe is highly stable in living cells and is capable of detecting changes in gene expression induced by external stimuli. Using dsLNA probes we then demonstrate the novel approaches to monitor the spatiotemporal gene expression response during cell injury. Our results also suggest a potential autoregulatory role of Nrf2 in injury induced EMT. We also show that the signaling level of dsLNA probe can serve as a molecular signature for the leader cells near the wound which allows us to track the behaviors of leader cells during collective cell migration. Finally multimodal GNR-LNA approach is proposed to map spatiotemporal gene expression profile and reveal dynamic characteristics of heat shock response in photothermal operations.
Type:
text; Electronic Dissertation
Keywords:
Gene expression; Optical molecular Probes; wound healing assays; Mechanical Engineering; collective cell migration
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Mechanical Engineering
Degree Grantor:
University of Arizona
Advisor:
Wong, Pak Kin

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleEngineered Molecular Probes for Systematic Studies of Cellular Response in Collective Cell Migrationen_US
dc.creatorRiahi, Rezaen_US
dc.contributor.authorRiahi, Rezaen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe investigation of complex biological processes, such as wound healing, cell migration, cancer cell invasion, and gene regulatory networks can be benefited tremendously by novel biosensing techniques with high stability and spatiotemporal resolution. In particular, molecular probes with qualities including high stability, sensitivity, and specificity are highly sought-after for long-term monitoring of gene expression in individual cells. Among different single-cell analysis techniques oligonucleotide optical probes is a promising detection method to monitor the dynamics of cellular responses. Herein, the design and optimization of double-stranded LNA probes are first investigated. With alternating DNA/LNA monomers for optimizing the stability and specificity, we show that the probe is highly stable in living cells and is capable of detecting changes in gene expression induced by external stimuli. Using dsLNA probes we then demonstrate the novel approaches to monitor the spatiotemporal gene expression response during cell injury. Our results also suggest a potential autoregulatory role of Nrf2 in injury induced EMT. We also show that the signaling level of dsLNA probe can serve as a molecular signature for the leader cells near the wound which allows us to track the behaviors of leader cells during collective cell migration. Finally multimodal GNR-LNA approach is proposed to map spatiotemporal gene expression profile and reveal dynamic characteristics of heat shock response in photothermal operations.en_US
dc.typetexten
dc.typeElectronic Dissertationen
dc.subjectGene expressionen_US
dc.subjectOptical molecular Probesen_US
dc.subjectwound healing assaysen_US
dc.subjectMechanical Engineeringen_US
dc.subjectcollective cell migrationen_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMechanical Engineeringen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWong, Pak Kinen_US
dc.contributor.committeememberWong, Pak Kinen_US
dc.contributor.committeememberZohar, Yitshaken_US
dc.contributor.committeememberWu, Xiaoyien_US
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