Detection and Attempted Quantification of Allelopathic Chemicals in Buffelgrass (Pennisetum ciliare) Root Leachates Using Reverse Phase-HPLC

Persistent Link:
http://hdl.handle.net/10150/297777
Title:
Detection and Attempted Quantification of Allelopathic Chemicals in Buffelgrass (Pennisetum ciliare) Root Leachates Using Reverse Phase-HPLC
Author:
Vo, Duyen Thuy
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Pennisetum ciliare, commonly known as buffelgrass, is an invasive grass that has led to noticeable declines in perennial diversity and abundance. Allelopathy, which describes the exudation of secondary metabolites that result in detrimental effects upon neighboring species, has been implicated as a possible mechanism for buffelgrass' success due to observations that its root leachates inhibit the growth of native plants. Since past researchers have only revealed the identity of the acids present within the leachates (Table 1), I have proposed methods in which to extract and quantify these chemicals by employing reverse phase-HPLC. By comparing retention times with that of standards for the identified allelochemicals, p-hydroxybenzoic, vanillic, syringic, and p-coumaric acid were confirmed in buffelgrass root leachates by HPLC. Using calibration curves, buffelgrass roots were found to consist of 5.801x10⁻³±5x10⁻⁶% p-hydroxybenzoic acid, 3.259x10⁻³±8x10⁻⁶% vanillic acid, 4.940x10⁻³±5x10⁻⁶% syringic acid, and 4.369x10⁻³±5x10⁻⁶% p-coumaric acid, by weight. The detection of the acids demonstrated that these compounds were at a significant enough concentration to be detected, thereby suggesting that they may play a major role in plant interference. By detecting and quantifying phytotoxins in native and invasive plants, the chemicals responsible for inhibiting plant growth could be elucidated, establishing a quantifiable criterion of allelopathy.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry
Degree Grantor:
University of Arizona
Advisor:
Dlugosch, Katrina

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDetection and Attempted Quantification of Allelopathic Chemicals in Buffelgrass (Pennisetum ciliare) Root Leachates Using Reverse Phase-HPLCen_US
dc.creatorVo, Duyen Thuyen_US
dc.contributor.authorVo, Duyen Thuyen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractPennisetum ciliare, commonly known as buffelgrass, is an invasive grass that has led to noticeable declines in perennial diversity and abundance. Allelopathy, which describes the exudation of secondary metabolites that result in detrimental effects upon neighboring species, has been implicated as a possible mechanism for buffelgrass' success due to observations that its root leachates inhibit the growth of native plants. Since past researchers have only revealed the identity of the acids present within the leachates (Table 1), I have proposed methods in which to extract and quantify these chemicals by employing reverse phase-HPLC. By comparing retention times with that of standards for the identified allelochemicals, p-hydroxybenzoic, vanillic, syringic, and p-coumaric acid were confirmed in buffelgrass root leachates by HPLC. Using calibration curves, buffelgrass roots were found to consist of 5.801x10⁻³±5x10⁻⁶% p-hydroxybenzoic acid, 3.259x10⁻³±8x10⁻⁶% vanillic acid, 4.940x10⁻³±5x10⁻⁶% syringic acid, and 4.369x10⁻³±5x10⁻⁶% p-coumaric acid, by weight. The detection of the acids demonstrated that these compounds were at a significant enough concentration to be detected, thereby suggesting that they may play a major role in plant interference. By detecting and quantifying phytotoxins in native and invasive plants, the chemicals responsible for inhibiting plant growth could be elucidated, establishing a quantifiable criterion of allelopathy.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorDlugosch, Katrina-
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