Persistent Link:
http://hdl.handle.net/10150/297714
Title:
The Regulation of Bile Acids on NHE8 Gene Expression
Author:
Nguyen, Elizabeth Ann Gaddi
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Sodium is an essential electrolyte necessary to keep multiple systems in the human body functional. Knowledge of sodium-proton exchanger will give rise to multiple clinical applications to diseases that affect our current population, which is why I personally am interested in conducting research in this field. In order to understand GW 4064 effect on NHE8 gene expression, protein and RNA expression were investigated via treatment at different concentrations and different time intervals through Western blotting and Real-Time PCR Analysis. To find the mechanism behind GW 4064 effects on NHE 8 expression, the PGL3 FXR promoter was utilized to see if GW 4064 inhibitory mechanism was through transcription or translation. Expression, stimulation, and inhibition were determined via numerical calculations. From the resulting data, we can conclude that GW 4064 inhibition of NHE8 protein and RNA expression occurs via transcription.
Type:
text; Electronic Thesis
Degree Name:
B.S.H.S.
Degree Level:
bachelors
Degree Program:
Honors College; Physiology
Degree Grantor:
University of Arizona
Advisor:
Xu, Hua

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleThe Regulation of Bile Acids on NHE8 Gene Expressionen_US
dc.creatorNguyen, Elizabeth Ann Gaddien_US
dc.contributor.authorNguyen, Elizabeth Ann Gaddien_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractSodium is an essential electrolyte necessary to keep multiple systems in the human body functional. Knowledge of sodium-proton exchanger will give rise to multiple clinical applications to diseases that affect our current population, which is why I personally am interested in conducting research in this field. In order to understand GW 4064 effect on NHE8 gene expression, protein and RNA expression were investigated via treatment at different concentrations and different time intervals through Western blotting and Real-Time PCR Analysis. To find the mechanism behind GW 4064 effects on NHE 8 expression, the PGL3 FXR promoter was utilized to see if GW 4064 inhibitory mechanism was through transcription or translation. Expression, stimulation, and inhibition were determined via numerical calculations. From the resulting data, we can conclude that GW 4064 inhibition of NHE8 protein and RNA expression occurs via transcription.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.H.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorXu, Hua-
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