Screen for Photoreceptor and Other Eyespot Defective Mutants in Chlamydomonas

Persistent Link:
http://hdl.handle.net/10150/297711
Title:
Screen for Photoreceptor and Other Eyespot Defective Mutants in Chlamydomonas
Author:
Moneleone, Victoria
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The purpose of this project was to test mutant strains of Chlamydomonas reinhardtii for a variation in eyespot location and size. The technique of phototaxis was used to determine if mutant strains have an altered ability of light reception. Cells from 96-well plates were transferred into the respective tubes filled with 2.2 mL of M medium. One row of tubes was placed in a test tube rack and two racks were placed in each phototaxis box. After twenty minutes in each box, where the only amount of light being let in is from a tiny slit directed on the sample, the tubes were removed and then checked for completion of phototaxis. After noting whether the samples completed phototaxis or not, the phototaxis-negative cells were regrown for further observation and ultimately a PCR screen for the mutant gene sequences. Out of roughly 5,000 mutant samples screened using phototaxis, twelve samples were phototaxis-negative and only four of the twelve samples were phototaxis-negative the second trial. Further experimentation would include the observation of the eyespots under a microscope, culturing these samples, harvesting the DNA via electroporation, and screening the samples using PCR.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Chemistry & Biochemistry
Degree Grantor:
University of Arizona
Advisor:
Dieckmann, Carol; Charest, Pascale

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleScreen for Photoreceptor and Other Eyespot Defective Mutants in Chlamydomonasen_US
dc.creatorMoneleone, Victoriaen_US
dc.contributor.authorMoneleone, Victoriaen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe purpose of this project was to test mutant strains of Chlamydomonas reinhardtii for a variation in eyespot location and size. The technique of phototaxis was used to determine if mutant strains have an altered ability of light reception. Cells from 96-well plates were transferred into the respective tubes filled with 2.2 mL of M medium. One row of tubes was placed in a test tube rack and two racks were placed in each phototaxis box. After twenty minutes in each box, where the only amount of light being let in is from a tiny slit directed on the sample, the tubes were removed and then checked for completion of phototaxis. After noting whether the samples completed phototaxis or not, the phototaxis-negative cells were regrown for further observation and ultimately a PCR screen for the mutant gene sequences. Out of roughly 5,000 mutant samples screened using phototaxis, twelve samples were phototaxis-negative and only four of the twelve samples were phototaxis-negative the second trial. Further experimentation would include the observation of the eyespots under a microscope, culturing these samples, harvesting the DNA via electroporation, and screening the samples using PCR.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineChemistry & Biochemistryen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorDieckmann, Carol-
dc.contributor.advisorCharest, Pascale-
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