Mapping and Characterizing the Neuropil-Associated Glia in Drosophila Larvae

Persistent Link:
http://hdl.handle.net/10150/297701
Title:
Mapping and Characterizing the Neuropil-Associated Glia in Drosophila Larvae
Author:
Lee, Si Woo
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In Drosophila central nervous system, neurons and glia collaborate on tasks that are essential for the survival and development of neural organization, such as energy metabolism and neurogenesis. Astrocyte-like glial cells closely associate with the synaptic region neuropil in the ventral nerve cord (VNC). To obtain a better understanding of their potential contribution to neuronal circuit function, the spatial relationship between neurons and glial cells must be characterized. In the first phase of this study, I determined the number of glial cells and their positions in two specific VNC segments (3rd thoracic and 1st abdominal) of 3rd instar Drosophila larvae by employing a genetically encoded fluorescent protein, GFP, targeted specifically to the glial cell bodies and labeling a Fasciclin II positive axons as reference points. The confocal image reconstructions revealed that each segment typically had ten glial cells and only about half of them were consistently observed. In the second phase, I used Flybow technique to induce the expression of different fluorescent proteins in glial cells and observed their branching pattern. These glial cells extended their branches in stereotyped positions in the neuropil suggesting that each glial cell occupies its own domain, consistent with findings for astrocytes in vertebrate cortex.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Oland, Lynne

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleMapping and Characterizing the Neuropil-Associated Glia in Drosophila Larvaeen_US
dc.creatorLee, Si Wooen_US
dc.contributor.authorLee, Si Wooen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn Drosophila central nervous system, neurons and glia collaborate on tasks that are essential for the survival and development of neural organization, such as energy metabolism and neurogenesis. Astrocyte-like glial cells closely associate with the synaptic region neuropil in the ventral nerve cord (VNC). To obtain a better understanding of their potential contribution to neuronal circuit function, the spatial relationship between neurons and glial cells must be characterized. In the first phase of this study, I determined the number of glial cells and their positions in two specific VNC segments (3rd thoracic and 1st abdominal) of 3rd instar Drosophila larvae by employing a genetically encoded fluorescent protein, GFP, targeted specifically to the glial cell bodies and labeling a Fasciclin II positive axons as reference points. The confocal image reconstructions revealed that each segment typically had ten glial cells and only about half of them were consistently observed. In the second phase, I used Flybow technique to induce the expression of different fluorescent proteins in glial cells and observed their branching pattern. These glial cells extended their branches in stereotyped positions in the neuropil suggesting that each glial cell occupies its own domain, consistent with findings for astrocytes in vertebrate cortex.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorOland, Lynne-
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