Effects of Constant Track Running Exercise on the Expression of the Immediate Early Gene Arc in the Rat Hippocampus

Persistent Link:
http://hdl.handle.net/10150/297635
Title:
Effects of Constant Track Running Exercise on the Expression of the Immediate Early Gene Arc in the Rat Hippocampus
Author:
Kumar, Sabhya
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Arc is an IEG expressed in the CA1 sub-field of the hippocampus, following stimulus from an external environment and is expressed in the place cells involved in spatial learning. An initial experiment showed that Arc expression levels decreased with 25 minute rest periods between sessions. The current study aimed to see if similar results are seen with track running, if the rest periods are removed. The rats ran on a small circular track, ensuring that a small ensemble of place cells activate on each lap. Animals were assigned to 4 groups: positive control for Arc induced by MECS; negative control for animals sacrificed from their home cage; a 5 minute group and a 45 minute group. The brain tissue was stained using FISH. The 45 minute group had a 4% lower Arc expression than the 5 minute group. A univariate ANOVA indicated a statistically significant effect in the main factor of Behavioral Group (F= 6.955, p=0.03). Tukey tests showed that the differences between the MECS and the CC groups (p<0.05) and the MECS and the 45 minute groups (p<0.05) were significant. The behavioral groups showed no statistical significance meaning that massed exposure without rest does not attenuate Arc signal.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Barnes, Carol A.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleEffects of Constant Track Running Exercise on the Expression of the Immediate Early Gene Arc in the Rat Hippocampusen_US
dc.creatorKumar, Sabhyaen_US
dc.contributor.authorKumar, Sabhyaen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractArc is an IEG expressed in the CA1 sub-field of the hippocampus, following stimulus from an external environment and is expressed in the place cells involved in spatial learning. An initial experiment showed that Arc expression levels decreased with 25 minute rest periods between sessions. The current study aimed to see if similar results are seen with track running, if the rest periods are removed. The rats ran on a small circular track, ensuring that a small ensemble of place cells activate on each lap. Animals were assigned to 4 groups: positive control for Arc induced by MECS; negative control for animals sacrificed from their home cage; a 5 minute group and a 45 minute group. The brain tissue was stained using FISH. The 45 minute group had a 4% lower Arc expression than the 5 minute group. A univariate ANOVA indicated a statistically significant effect in the main factor of Behavioral Group (F= 6.955, p=0.03). Tukey tests showed that the differences between the MECS and the CC groups (p<0.05) and the MECS and the 45 minute groups (p<0.05) were significant. The behavioral groups showed no statistical significance meaning that massed exposure without rest does not attenuate Arc signal.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBarnes, Carol A.-
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