Visualizing an RB-E2F Cellular Switch that Controls Cell Proliferation

Persistent Link:
http://hdl.handle.net/10150/297627
Title:
Visualizing an RB-E2F Cellular Switch that Controls Cell Proliferation
Author:
Khav, Eddie
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Mammalian cell proliferation is regulated by an Rb-E2F gene network. The input node of this network, Cyclin D, receives graded growth signals; the output node, E2F, generates an all-or-none response. That is, the Rb-E2F gene network functions as a cellular switch, converting analog growth signals into digital E2F activities. The On or Off of this Rb-E2F switch determines the On or Off of cell proliferation. To help better understand the analog/digital conversion mechanism, we constructed a reporter cell line to visualize the dynamic expression of Cyclin D and E2F genes by red and green fluorescence in individual cells, respectively.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry
Degree Grantor:
University of Arizona
Advisor:
Yao, Guang

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleVisualizing an RB-E2F Cellular Switch that Controls Cell Proliferationen_US
dc.creatorKhav, Eddieen_US
dc.contributor.authorKhav, Eddieen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractMammalian cell proliferation is regulated by an Rb-E2F gene network. The input node of this network, Cyclin D, receives graded growth signals; the output node, E2F, generates an all-or-none response. That is, the Rb-E2F gene network functions as a cellular switch, converting analog growth signals into digital E2F activities. The On or Off of this Rb-E2F switch determines the On or Off of cell proliferation. To help better understand the analog/digital conversion mechanism, we constructed a reporter cell line to visualize the dynamic expression of Cyclin D and E2F genes by red and green fluorescence in individual cells, respectively.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorYao, Guang-
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