Persistent Link:
http://hdl.handle.net/10150/297618
Title:
Quantification of Pentosidine in Avian Plasma
Author:
Gu, Mary
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Sustained elevated plasma glucose concentrations, such as occurs in diabetes, leads to the non-enzymatic binding of glucose to serum albumin forming glycated albumin. Subsequent chemical rearrangement leads to the formation of irreversible Advanced Glycation Endproducts (AGEs). These AGEs can cause severe complications of diabetes such as microvascular and renal damage. It has been demonstrated that birds, as a normal aspect of their physiology, have plasma glucose concentrations of 350 mg/dL or higher. This concentration is about three times higher than that of mammals. This suggests that birds may have higher concentrations of AGEs in their plasma compared to mammals. However, we suggest that there is not a positive relationship between pentosidine and glucose levels in avian plasma, which would explain the absence of hyperglycemic complications. Pentosidine is a well-characterized AGE, stable under acid hydrolysis and has a lysine and arginine fluorescent cross link, allowing it to be easily detectable at low concentrations using High Performance Liquid Chromatography. Because of the amino groups available on pentosidine, we found that it could be easily detected using the Ninhydrin assay. Our results revealed an average pentosidine concentration of 0.032 pmol/ul and average glucose concentration of 390 pmol/ul in avian plasma.
Type:
text; Electronic Thesis
Degree Name:
B.H.S.
Degree Level:
bachelors
Degree Program:
Honors College; Physiology
Degree Grantor:
University of Arizona
Advisor:
Braun, Eldon

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleQuantification of Pentosidine in Avian Plasmaen_US
dc.creatorGu, Maryen_US
dc.contributor.authorGu, Maryen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractSustained elevated plasma glucose concentrations, such as occurs in diabetes, leads to the non-enzymatic binding of glucose to serum albumin forming glycated albumin. Subsequent chemical rearrangement leads to the formation of irreversible Advanced Glycation Endproducts (AGEs). These AGEs can cause severe complications of diabetes such as microvascular and renal damage. It has been demonstrated that birds, as a normal aspect of their physiology, have plasma glucose concentrations of 350 mg/dL or higher. This concentration is about three times higher than that of mammals. This suggests that birds may have higher concentrations of AGEs in their plasma compared to mammals. However, we suggest that there is not a positive relationship between pentosidine and glucose levels in avian plasma, which would explain the absence of hyperglycemic complications. Pentosidine is a well-characterized AGE, stable under acid hydrolysis and has a lysine and arginine fluorescent cross link, allowing it to be easily detectable at low concentrations using High Performance Liquid Chromatography. Because of the amino groups available on pentosidine, we found that it could be easily detected using the Ninhydrin assay. Our results revealed an average pentosidine concentration of 0.032 pmol/ul and average glucose concentration of 390 pmol/ul in avian plasma.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.H.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBraun, Eldon-
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