Persistent Link:
http://hdl.handle.net/10150/297561
Title:
Study of Methods for DNA Extractions from Feathers
Author:
Feely, Cassandra Lynn
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Feathers have been used as a source of DNA for two decades. Feathers are made of keratinized cells which can make obtaining DNA from them difficult. This study compared three established methods of extracting DNA from feathers to ascertain if any produced a greater quantity of DNA. Each of the methods were conducted at five different time intervals (n=45). Each was a modification of the Qiagen DNeasy DNA extraction kit. A two way ANOVA determined if there was any differences among times or methods, and showed a significance difference among methods (F= 45.67, P < 0.001) but not times. A box-and-whisker plot was used to ascertain the most effective method. A second experiment employed the superior method to see if a trend of increasing DNA concentration with increasing time, suggested by first experiment's data, was real. This experiment was powered up to assure sufficient ability to detect differences among 3 sample sets if they existed (n=45). These results came back with no significance. This research found the extraction method used in Dr. Lisette Wait’s laboratory superior and has resulted in a protocol change for all extractions done in Dr. Melanie Culver's ancient DNA laboratory at the University of Arizona.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Culver, Melanie

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleStudy of Methods for DNA Extractions from Feathersen_US
dc.creatorFeely, Cassandra Lynnen_US
dc.contributor.authorFeely, Cassandra Lynnen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractFeathers have been used as a source of DNA for two decades. Feathers are made of keratinized cells which can make obtaining DNA from them difficult. This study compared three established methods of extracting DNA from feathers to ascertain if any produced a greater quantity of DNA. Each of the methods were conducted at five different time intervals (n=45). Each was a modification of the Qiagen DNeasy DNA extraction kit. A two way ANOVA determined if there was any differences among times or methods, and showed a significance difference among methods (F= 45.67, P < 0.001) but not times. A box-and-whisker plot was used to ascertain the most effective method. A second experiment employed the superior method to see if a trend of increasing DNA concentration with increasing time, suggested by first experiment's data, was real. This experiment was powered up to assure sufficient ability to detect differences among 3 sample sets if they existed (n=45). These results came back with no significance. This research found the extraction method used in Dr. Lisette Wait’s laboratory superior and has resulted in a protocol change for all extractions done in Dr. Melanie Culver's ancient DNA laboratory at the University of Arizona.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorCulver, Melanie-
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