TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae

Persistent Link:
http://hdl.handle.net/10150/297540
Title:
TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
Author:
Fanger, Christopher David
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
An abnormally large plasmid has been discovered to naturally exist in two strains of Pseudomonas syringae, appropriately called the "Megaplasmid" due to the genetic content of over one million base pairs of DNA. Costs have been observed with the presence of the Megaplasmid, including growth at elevated temperatures. A technique called Tn5 mutagenesis has been utilized to ameliorate the cost to the host. Results show the initial phenotype of Tn5 mutants overcame elevated temperature growth inhibition, but presence of another mutation is likely in order for the strain to grow at elevated temperatures while harboring the Megaplasmid. Further research must be done to identify genes responsible for increased fitness.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Maltrus, David A.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleTNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringaeen_US
dc.creatorFanger, Christopher Daviden_US
dc.contributor.authorFanger, Christopher Daviden_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAn abnormally large plasmid has been discovered to naturally exist in two strains of Pseudomonas syringae, appropriately called the "Megaplasmid" due to the genetic content of over one million base pairs of DNA. Costs have been observed with the presence of the Megaplasmid, including growth at elevated temperatures. A technique called Tn5 mutagenesis has been utilized to ameliorate the cost to the host. Results show the initial phenotype of Tn5 mutants overcame elevated temperature growth inhibition, but presence of another mutation is likely in order for the strain to grow at elevated temperatures while harboring the Megaplasmid. Further research must be done to identify genes responsible for increased fitness.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorMaltrus, David A.-
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