Comparative Analysis of Na-K-ATPase Protein Expression Levels in the Outer Medullary Portion of the Kangaroo Rat and the Sprague Dawley Rat Kidney

Persistent Link:
http://hdl.handle.net/10150/297510
Title:
Comparative Analysis of Na-K-ATPase Protein Expression Levels in the Outer Medullary Portion of the Kangaroo Rat and the Sprague Dawley Rat Kidney
Author:
Armstrong, Tamara Marie
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Certain proteins in the thick ascending limb of the nephron are thought to help create the urine concentration gradient in mammalian kidneys. Kangaroo rats have very concentrated urine because of their dry environment and thus make a useful species for comparison against the normal laboratory rat. Concentrations of NaK-ATPase were measured using Western Blot Analyses. The tissue was dissected to isolate the outer medullary region, which consists predominantly of the thick ascending limb. The membrane fraction of the tissue was blotted with an antibody against the NaK-ATPase alpha subunit and read with an infrared imaging system. The results showed that NaK-ATPase protein levels were over three fold higher in the kangaroo rat relative to the Sprague Dawley rat, supporting the hypothesis that more active sodium reabsorption occurs in the kangaroo rat. The release of inorganic phosphate was also measured to determine ouabain-sensitive NaK-ATPase activity. Total ATPase activity was 905 ± 122 for kangaroo rat and 721 ± 111 for Munich-Wistar rat (Mean ± SE). NaK-ATPase activity was greater in the kangaroo rat (204 ± 65) than in Munich-Wistar rat (79 ± 62).
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Physiology
Degree Grantor:
University of Arizona
Advisor:
Pannabecker, Thomas

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleComparative Analysis of Na-K-ATPase Protein Expression Levels in the Outer Medullary Portion of the Kangaroo Rat and the Sprague Dawley Rat Kidneyen_US
dc.creatorArmstrong, Tamara Marieen_US
dc.contributor.authorArmstrong, Tamara Marieen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractCertain proteins in the thick ascending limb of the nephron are thought to help create the urine concentration gradient in mammalian kidneys. Kangaroo rats have very concentrated urine because of their dry environment and thus make a useful species for comparison against the normal laboratory rat. Concentrations of NaK-ATPase were measured using Western Blot Analyses. The tissue was dissected to isolate the outer medullary region, which consists predominantly of the thick ascending limb. The membrane fraction of the tissue was blotted with an antibody against the NaK-ATPase alpha subunit and read with an infrared imaging system. The results showed that NaK-ATPase protein levels were over three fold higher in the kangaroo rat relative to the Sprague Dawley rat, supporting the hypothesis that more active sodium reabsorption occurs in the kangaroo rat. The release of inorganic phosphate was also measured to determine ouabain-sensitive NaK-ATPase activity. Total ATPase activity was 905 ± 122 for kangaroo rat and 721 ± 111 for Munich-Wistar rat (Mean ± SE). NaK-ATPase activity was greater in the kangaroo rat (204 ± 65) than in Munich-Wistar rat (79 ± 62).en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorPannabecker, Thomas-
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