Regulation of Runx2 and Other Downstream Factors in the TGFβ Pathway in Cardiac Epithelial to Mesenchymal Transition and Disease

Persistent Link:
http://hdl.handle.net/10150/297505
Title:
Regulation of Runx2 and Other Downstream Factors in the TGFβ Pathway in Cardiac Epithelial to Mesenchymal Transition and Disease
Author:
Acharya Shree
Issue Date:
2013
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Epithelial-mesenchymal transition (EMT) transforms developing embryonic endothelial cells of the heart into mesenchymal cells in the process of activation of downstream regulators of the TGFβ pathway, a major component in EMT signaling, such as the positive transcription factor, Runx2. EMT has been shown to occur in pathogenic, cardiac diseases in the adult as well as in developmental embryonic situations. Here, we explore the Runx2 isoforms and their role in each of these processes, as well as the roles of Runx2’s inhibition on downstream transcription and intracellular (stem-cell possessing) factors, Zeb2 and ALDH1, respectively. We concluded that Runx2 isoforms show a relationship in the calcification of the heart, and that Runx2-I expression is also in the embryonic stages. Also, TGFβ-2 induces Runx2 expression, while silencing RNA against Runx2 reduces its prominence, and essentially induces expression of Zeb2 and ALDH1 through a limited regulation. By deciphering the roles of these fundamental yet intricate players and mechanisms in EMT, a gateway to deciphering potential target therapeutics will also be prominent in future developments for patients with congenital cardiac disease.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biology
Degree Grantor:
University of Arizona
Advisor:
Runyan, Raymond

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleRegulation of Runx2 and Other Downstream Factors in the TGFβ Pathway in Cardiac Epithelial to Mesenchymal Transition and Diseaseen_US
dc.creatorAcharya Shreeen_US
dc.contributor.authorAcharya Shreeen_US
dc.date.issued2013-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractEpithelial-mesenchymal transition (EMT) transforms developing embryonic endothelial cells of the heart into mesenchymal cells in the process of activation of downstream regulators of the TGFβ pathway, a major component in EMT signaling, such as the positive transcription factor, Runx2. EMT has been shown to occur in pathogenic, cardiac diseases in the adult as well as in developmental embryonic situations. Here, we explore the Runx2 isoforms and their role in each of these processes, as well as the roles of Runx2’s inhibition on downstream transcription and intracellular (stem-cell possessing) factors, Zeb2 and ALDH1, respectively. We concluded that Runx2 isoforms show a relationship in the calcification of the heart, and that Runx2-I expression is also in the embryonic stages. Also, TGFβ-2 induces Runx2 expression, while silencing RNA against Runx2 reduces its prominence, and essentially induces expression of Zeb2 and ALDH1 through a limited regulation. By deciphering the roles of these fundamental yet intricate players and mechanisms in EMT, a gateway to deciphering potential target therapeutics will also be prominent in future developments for patients with congenital cardiac disease.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorRunyan, Raymond-
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