Isolation and characterization of a vacuolar Na⁺/H⁺ exchanger in Salicornia bigelovii Torr.

Persistent Link:
http://hdl.handle.net/10150/292070
Title:
Isolation and characterization of a vacuolar Na⁺/H⁺ exchanger in Salicornia bigelovii Torr.
Author:
Kshirsagar, Meenakshi K.
Issue Date:
2002
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The work in this thesis provided critical information about the vacuolar Na⁺/H⁺ exchanger in Salicornia bigelovii with a goal to understand its role in plant salt tolerance. A cDNA (SbNHX1) was isolated and showed 87% identity to the Na⁺/H⁺ exchanger from the glycophyte, Arabidopsis thaliana. DNA blot analysis suggests the presence of a family of Na⁺/H⁺ exchangers in Salicornia bigelovii . An increase in SbNHX1 transcript was seen in shoots of 200 mM NaCl-grown plants compared to transcript levels in 5 mM NaCI-grown plants. To understand whether the SbNHX1 cDNA conferred increased salt tolerance by virtue of its unique sequence, the coding region of SbNHX1 was cloned for expression in Arabidopsis thaliana . Potential Ca⁺² regulation of vacuolar Na⁺/H⁺ exchange is shown using transport experiments. The isolation of a vacuolar Na+/H+ exchanger from Salicornia bigelovii and its functional expression in Arabidopsis thaliana will provide information on the salt tolerance mechanisms used by halophytes. (Abstract shortened by UMI.)
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Agriculture, Plant Culture.; Biology, Plant Physiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Plant Sciences
Degree Grantor:
University of Arizona
Advisor:
Schumaker, Karen S.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleIsolation and characterization of a vacuolar Na⁺/H⁺ exchanger in Salicornia bigelovii Torr.en_US
dc.creatorKshirsagar, Meenakshi K.en_US
dc.contributor.authorKshirsagar, Meenakshi K.en_US
dc.date.issued2002en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe work in this thesis provided critical information about the vacuolar Na⁺/H⁺ exchanger in Salicornia bigelovii with a goal to understand its role in plant salt tolerance. A cDNA (SbNHX1) was isolated and showed 87% identity to the Na⁺/H⁺ exchanger from the glycophyte, Arabidopsis thaliana. DNA blot analysis suggests the presence of a family of Na⁺/H⁺ exchangers in Salicornia bigelovii . An increase in SbNHX1 transcript was seen in shoots of 200 mM NaCl-grown plants compared to transcript levels in 5 mM NaCI-grown plants. To understand whether the SbNHX1 cDNA conferred increased salt tolerance by virtue of its unique sequence, the coding region of SbNHX1 was cloned for expression in Arabidopsis thaliana . Potential Ca⁺² regulation of vacuolar Na⁺/H⁺ exchange is shown using transport experiments. The isolation of a vacuolar Na+/H+ exchanger from Salicornia bigelovii and its functional expression in Arabidopsis thaliana will provide information on the salt tolerance mechanisms used by halophytes. (Abstract shortened by UMI.)en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectAgriculture, Plant Culture.en_US
dc.subjectBiology, Plant Physiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorSchumaker, Karen S.en_US
dc.identifier.proquest1411698en_US
dc.identifier.bibrecord.b43472072en_US
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