Persistent Link:
http://hdl.handle.net/10150/291647
Title:
Temperature-regulated proteins in plants
Author:
Willett, Deanna Allyn
Issue Date:
1999
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Studies in this thesis concern expression of one class of small HSPs (sHSPs) in field grown desert plant species and the isolation of a new HSP gene encoding an sHSP targeted to plant mitochondria. Expression of class I, cytosolic sHSPs was assessed in three desert species: Screwbean Mesquite, Baja Fairyduster, and Sweet Acacia. Total leaf protein, and if available, flower and pod protein, was extracted from samples and analyzed by SDS-PAGE and Western blotting. Sweet Acacia showed strong sHSP expression in leaves with an apparent diurnal pattern of increased expression in the hotter PM. Screwbean Mesquite pods showed significant sHSP expression, which was not correlated to temperature. The isolation and sequence analysis of a gene encoding a mitochondrion-localized sHSP from Arabidopsis was completed. Comparisons to other plant sHSPs verified it was most similar to other mitochondrial-localized sHSPs from plants.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Chemistry, Biochemistry.; Biology, Plant Physiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Biochemistry
Degree Grantor:
University of Arizona
Advisor:
Vierling, Elizabeth

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleTemperature-regulated proteins in plantsen_US
dc.creatorWillett, Deanna Allynen_US
dc.contributor.authorWillett, Deanna Allynen_US
dc.date.issued1999en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractStudies in this thesis concern expression of one class of small HSPs (sHSPs) in field grown desert plant species and the isolation of a new HSP gene encoding an sHSP targeted to plant mitochondria. Expression of class I, cytosolic sHSPs was assessed in three desert species: Screwbean Mesquite, Baja Fairyduster, and Sweet Acacia. Total leaf protein, and if available, flower and pod protein, was extracted from samples and analyzed by SDS-PAGE and Western blotting. Sweet Acacia showed strong sHSP expression in leaves with an apparent diurnal pattern of increased expression in the hotter PM. Screwbean Mesquite pods showed significant sHSP expression, which was not correlated to temperature. The isolation and sequence analysis of a gene encoding a mitochondrion-localized sHSP from Arabidopsis was completed. Comparisons to other plant sHSPs verified it was most similar to other mitochondrial-localized sHSPs from plants.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectChemistry, Biochemistry.en_US
dc.subjectBiology, Plant Physiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorVierling, Elizabethen_US
dc.identifier.proquest1396514en_US
dc.identifier.bibrecord.b39915621en_US
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