Persistent Link:
http://hdl.handle.net/10150/291616
Title:
Influence of manganese on amylase gene expression
Author:
Chang, Siu-Chi, 1962-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Previous studies have suggested that manganese (Mn) deficiency is associated with increased pancreatic amylase activity in rats. The present study investigated whether this increase in amylase activity is a result of increased pancreatic amylase messenger RNA (mRNA) levels. Weanling rats were fed a high carbohydrate diet containing either 39.6 ppm (control) or 0.5 ppm (deficient) manganese for 4 to 8 weeks. Manganese deficiency was confirmed by determining hepatic manganese content which was significantly lower in Mn-deficient rats than in the respective controls. Pancreatic RNA was size-fractionated on formaldehyde gels, and hybridized with 32P-labeled complementary DNAs (cDNA) for amylase and trypsinogen. Amylase mRNA levels were increased significantly in both 4 week (200%) and 8 week (250%) Mn-deficient rats when compared with their respective controls. In contrast, manganese deficiency was not associated with alternations in trypsinogen mRNA levels. Moreover, in vitro translation of the pancreatic mRNA indicated that manganese deficiency increased amylase mRNA levels supporting the Northern Blot analysis. Insulin and corticosterone, hormones known to increase amylase mRNA levels, were not affected by Mn-deficiency. These observations suggest that manganese may participate in the regulation of amylase gene expression.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Manganese.; Amylases -- Synthesis.; Gene expression.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Nutritional Sciences
Degree Grantor:
University of Arizona
Advisor:
Korc, Murray

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleInfluence of manganese on amylase gene expressionen_US
dc.creatorChang, Siu-Chi, 1962-en_US
dc.contributor.authorChang, Siu-Chi, 1962-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractPrevious studies have suggested that manganese (Mn) deficiency is associated with increased pancreatic amylase activity in rats. The present study investigated whether this increase in amylase activity is a result of increased pancreatic amylase messenger RNA (mRNA) levels. Weanling rats were fed a high carbohydrate diet containing either 39.6 ppm (control) or 0.5 ppm (deficient) manganese for 4 to 8 weeks. Manganese deficiency was confirmed by determining hepatic manganese content which was significantly lower in Mn-deficient rats than in the respective controls. Pancreatic RNA was size-fractionated on formaldehyde gels, and hybridized with 32P-labeled complementary DNAs (cDNA) for amylase and trypsinogen. Amylase mRNA levels were increased significantly in both 4 week (200%) and 8 week (250%) Mn-deficient rats when compared with their respective controls. In contrast, manganese deficiency was not associated with alternations in trypsinogen mRNA levels. Moreover, in vitro translation of the pancreatic mRNA indicated that manganese deficiency increased amylase mRNA levels supporting the Northern Blot analysis. Insulin and corticosterone, hormones known to increase amylase mRNA levels, were not affected by Mn-deficiency. These observations suggest that manganese may participate in the regulation of amylase gene expression.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectManganese.en_US
dc.subjectAmylases -- Synthesis.en_US
dc.subjectGene expression.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineNutritional Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorKorc, Murrayen_US
dc.identifier.proquest1338842en_US
dc.identifier.oclc24081806en_US
dc.identifier.bibrecord.b17806252en_US
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