Persistent Link:
http://hdl.handle.net/10150/291433
Title:
Bacterial attachment in porous media
Author:
Hilbert, Thomas Adams, 1961-
Issue Date:
1992
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Colloid filtration theory was utilized to estimate the sticking coefficient (α) of bacteria in filter media. Determination of bacterial cell numbers was facilitated by incorporation of [³H] leucine into cells prior to filtration. Large changes in retention of bacteria within porous material correlated with different stages in the bacterial growth cycle. This was due primarily to changes in cell size and not due to a change in α. The effects of ionic strength, pH, nutrient status, surfactant concentration and filter material on α were also evaluated. Various filtration models predicted similar trends in the magnitude of bacterial α with changes in experimental conditions. Experiments were performed with two gram negative and one gram positive bacterial species, Pseudomonas fluorescens, Pseudomonas JS6, and Bacillus pumilus. Small reductions in α were observed in cultures that were carbon-limited. Oxygen limitation produced no change in alpha. Bacterial α's were a function of ionic strength and filter material.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Environmental Sciences.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Civil Engineering and Engineering Mechanics
Degree Grantor:
University of Arizona
Advisor:
Logan, Bruce E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleBacterial attachment in porous mediaen_US
dc.creatorHilbert, Thomas Adams, 1961-en_US
dc.contributor.authorHilbert, Thomas Adams, 1961-en_US
dc.date.issued1992en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractColloid filtration theory was utilized to estimate the sticking coefficient (α) of bacteria in filter media. Determination of bacterial cell numbers was facilitated by incorporation of [³H] leucine into cells prior to filtration. Large changes in retention of bacteria within porous material correlated with different stages in the bacterial growth cycle. This was due primarily to changes in cell size and not due to a change in α. The effects of ionic strength, pH, nutrient status, surfactant concentration and filter material on α were also evaluated. Various filtration models predicted similar trends in the magnitude of bacterial α with changes in experimental conditions. Experiments were performed with two gram negative and one gram positive bacterial species, Pseudomonas fluorescens, Pseudomonas JS6, and Bacillus pumilus. Small reductions in α were observed in cultures that were carbon-limited. Oxygen limitation produced no change in alpha. Bacterial α's were a function of ionic strength and filter material.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectEnvironmental Sciences.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineCivil Engineering and Engineering Mechanicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLogan, Bruce E.en_US
dc.identifier.proquest1350943en_US
dc.identifier.bibrecord.b26422529en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.