Persistent Link:
http://hdl.handle.net/10150/289778
Title:
Glycine receptors in the developing rat spinal cord
Author:
Kumar, David Vijay
Issue Date:
2001
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Inhibitory glycine receptors (GlyRs) undergo developmental regulation with α2 subunits most abundant at prenatal and early postnatal stages, and α1 subunits predominating in adults. In comparing the relative amounts of mRNA for two known splice variants of the α2 subunit, α2A and α2b, in the rat spinal cord at different stages of development, I found evidence for the existence of an additional, novel variant. This variant, missing exon 3, I have termed "α2N." Examination of the RNA from spinal cords of different-aged rats indicated that α2N undergoes dramatic down-regulation during prenatal development. Its mRNA forms a significant portion of the α2 subunit pool at E14, but is nearly undetectable at the time of birth. I also examined the developmental changes in two factors that can regulate GlyR α2 pre-mRNA splicing, the splicing factor neurooncological ventral antigen-1 (Nova-1) and the brain isoform of the polypyrimidine tract binding protein (brPTB). Treatment of neurons in culture with antisense oligonucleotides to "knock down" one of the Nova-1 variants altered the expression of GlyR α2N. These results suggest that the relative levels of the variants of Nova-1 and brPTB may play a role in the developmental regulation of GlyR α2N. Based on these results I propose a model for the developmental regulation of GlyR α2N. These results provide evidence for a novel splice variant of the GlyR α2 subunit that undergoes dramatic developmental regulation, and reveal the developmental profiles of two possible regulators of its expression during development.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Biology, Anatomy.; Biology, Neuroscience.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Cell Biology and Anatomy
Degree Grantor:
University of Arizona
Advisor:
St. John, Paul A.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleGlycine receptors in the developing rat spinal corden_US
dc.creatorKumar, David Vijayen_US
dc.contributor.authorKumar, David Vijayen_US
dc.date.issued2001en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractInhibitory glycine receptors (GlyRs) undergo developmental regulation with α2 subunits most abundant at prenatal and early postnatal stages, and α1 subunits predominating in adults. In comparing the relative amounts of mRNA for two known splice variants of the α2 subunit, α2A and α2b, in the rat spinal cord at different stages of development, I found evidence for the existence of an additional, novel variant. This variant, missing exon 3, I have termed "α2N." Examination of the RNA from spinal cords of different-aged rats indicated that α2N undergoes dramatic down-regulation during prenatal development. Its mRNA forms a significant portion of the α2 subunit pool at E14, but is nearly undetectable at the time of birth. I also examined the developmental changes in two factors that can regulate GlyR α2 pre-mRNA splicing, the splicing factor neurooncological ventral antigen-1 (Nova-1) and the brain isoform of the polypyrimidine tract binding protein (brPTB). Treatment of neurons in culture with antisense oligonucleotides to "knock down" one of the Nova-1 variants altered the expression of GlyR α2N. These results suggest that the relative levels of the variants of Nova-1 and brPTB may play a role in the developmental regulation of GlyR α2N. Based on these results I propose a model for the developmental regulation of GlyR α2N. These results provide evidence for a novel splice variant of the GlyR α2 subunit that undergoes dramatic developmental regulation, and reveal the developmental profiles of two possible regulators of its expression during development.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBiology, Anatomy.en_US
dc.subjectBiology, Neuroscience.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineCell Biology and Anatomyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorSt. John, Paul A.en_US
dc.identifier.proquest3010202en_US
dc.identifier.bibrecord.b4161141xen_US
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