Localization and molecular signaling pathways of prostaglandin receptor subtypes in the eye

Persistent Link:
http://hdl.handle.net/10150/282743
Title:
Localization and molecular signaling pathways of prostaglandin receptor subtypes in the eye
Author:
Anthony, Todd Lee, 1965-
Issue Date:
1998
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The eicosanoids are derived from membrane fatty acids. Prostaglandins (PGs), produced from the metabolism of arachidonic acid, are members of the eicosanoid family. Prostaglandins exert a broad range of biological effects by interacting with plasma membrane-bound receptors that are coupled to guanine nucleotide-binding proteins (G proteins). The topical application of prostaglandins results in a long-lasting reduction in intraocular pressure (IOP) in mammalian eyes, including humans. The distribution and function of the prostaglandin receptor subtypes within the eye are not clearly understood. The studies presented in this dissertation are focused at addressing three specific aims which have been designed to test the following hypothesis; that prostaglandin receptors are involved in the maintenance of intraocular pressure through the modulation of aqueous humor production and in the regulation of aqueous humor outflow pathways. Subtype selective antibodies to the individual prostanoid receptors have been generated to enable the study of these receptors at the tissue, cellular and molecular level. Utilization of the antibodies and a series of pharmacological and molecular techniques have identified specific prostanoid receptor subtypes in areas of the eye which are involved in the regulation of IOP. The functional responses obtained in primary cultures of human trabecular meshwork and bovine ciliary epithelium provide evidence for the involvement of selective prostanoid receptor subtypes in the regulation of both aqueous humor production and aqueous humor outflow.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Biology, Molecular.; Health Sciences, Pharmacology.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Pharmacology and Toxicology
Degree Grantor:
University of Arizona
Advisor:
Regan, John W.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleLocalization and molecular signaling pathways of prostaglandin receptor subtypes in the eyeen_US
dc.creatorAnthony, Todd Lee, 1965-en_US
dc.contributor.authorAnthony, Todd Lee, 1965-en_US
dc.date.issued1998en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe eicosanoids are derived from membrane fatty acids. Prostaglandins (PGs), produced from the metabolism of arachidonic acid, are members of the eicosanoid family. Prostaglandins exert a broad range of biological effects by interacting with plasma membrane-bound receptors that are coupled to guanine nucleotide-binding proteins (G proteins). The topical application of prostaglandins results in a long-lasting reduction in intraocular pressure (IOP) in mammalian eyes, including humans. The distribution and function of the prostaglandin receptor subtypes within the eye are not clearly understood. The studies presented in this dissertation are focused at addressing three specific aims which have been designed to test the following hypothesis; that prostaglandin receptors are involved in the maintenance of intraocular pressure through the modulation of aqueous humor production and in the regulation of aqueous humor outflow pathways. Subtype selective antibodies to the individual prostanoid receptors have been generated to enable the study of these receptors at the tissue, cellular and molecular level. Utilization of the antibodies and a series of pharmacological and molecular techniques have identified specific prostanoid receptor subtypes in areas of the eye which are involved in the regulation of IOP. The functional responses obtained in primary cultures of human trabecular meshwork and bovine ciliary epithelium provide evidence for the involvement of selective prostanoid receptor subtypes in the regulation of both aqueous humor production and aqueous humor outflow.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectHealth Sciences, Pharmacology.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePharmacology and Toxicologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorRegan, John W.en_US
dc.identifier.proquest9906510en_US
dc.identifier.bibrecord.b38862384en_US
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