Identification and partial characterization of two chloroplast-encoded potential RNA metabolism genes roaA and mat1

Persistent Link:
http://hdl.handle.net/10150/282114
Title:
Identification and partial characterization of two chloroplast-encoded potential RNA metabolism genes roaA and mat1
Author:
Jenkins, Kristin Perth, 1965-
Issue Date:
1996
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Although group I and group II introns are capable of self-splicing in vitro, there is growing evidence that trans acting factors facilitate splicing of group I and group II introns in vivo. For group II introns, these trans acting factors include maturases and RNA chaperones. Maturases are intron-encoded enzymes which splice the intron which encodes them and in some cases mobilize the intron. RNA chaperones are a group of general RNA binding proteins which facilitate formation of correctly folded catalytic RNAs. The Euglena gracilis chloroplast genome is an ideal system in which to study RNA metabolism, especially splicing. The genome is composed of 38% introns, including group II, group III and twintrons. Although Euglena gracilis chloroplast pre-mRNAs must undergo extensive processing and splicing before they are translated, no chloroplast encoded maturases or RNA chaperones have been described. I have characterized two potential RNA metabolism genes. The roaA gene is unique to the Euglena chloroplast and encodes a potential general RNA binding protein. The roaA gene and mRNA are defined in this dissertation. The roaA protein product and the protein product of a potential maturase, mat1, have also been identified. Identification of these two protein products lays the groundwork for future studies regarding the role of these two proteins in chloroplast RNA metabolism.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Biology, Molecular.; Biology, Cell.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Hallick, Richard B.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleIdentification and partial characterization of two chloroplast-encoded potential RNA metabolism genes roaA and mat1en_US
dc.creatorJenkins, Kristin Perth, 1965-en_US
dc.contributor.authorJenkins, Kristin Perth, 1965-en_US
dc.date.issued1996en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAlthough group I and group II introns are capable of self-splicing in vitro, there is growing evidence that trans acting factors facilitate splicing of group I and group II introns in vivo. For group II introns, these trans acting factors include maturases and RNA chaperones. Maturases are intron-encoded enzymes which splice the intron which encodes them and in some cases mobilize the intron. RNA chaperones are a group of general RNA binding proteins which facilitate formation of correctly folded catalytic RNAs. The Euglena gracilis chloroplast genome is an ideal system in which to study RNA metabolism, especially splicing. The genome is composed of 38% introns, including group II, group III and twintrons. Although Euglena gracilis chloroplast pre-mRNAs must undergo extensive processing and splicing before they are translated, no chloroplast encoded maturases or RNA chaperones have been described. I have characterized two potential RNA metabolism genes. The roaA gene is unique to the Euglena chloroplast and encodes a potential general RNA binding protein. The roaA gene and mRNA are defined in this dissertation. The roaA protein product and the protein product of a potential maturase, mat1, have also been identified. Identification of these two protein products lays the groundwork for future studies regarding the role of these two proteins in chloroplast RNA metabolism.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectBiology, Cell.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorHallick, Richard B.en_US
dc.identifier.proquest9706151en_US
dc.identifier.bibrecord.b34263159en_US
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