Persistent Link:
http://hdl.handle.net/10150/282091
Title:
Characterization of T4 tertiary origins
Author:
Menkens, Anne Elizabeth, 1958-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The bacteriophage T4 utilizes at least three modes of initiation of replication, termed primary, secondary and tertiary (Mosig, 1983; Kreuzer and Alberts, 1985). Two origins of replication have been isolated that utilize the tertiary mode of initiation. The DNA sequence requirements of the two tertiary origins have been characterized at the nucleotide level. Maximal replication of each origin-containing plasmid required both an intact gpmotA-dependent middle-mode promoter sequence and approximately 50 basepairs of the downstream region. In contrast, gpmotA-dependent transcription from the origin promoter was found to be independent of the downstream region. The requirement for a promoter element within the tertiary origins is striking, particularly since the replication of tertiary origin-containing plasmids is resistant to the RNA polymerase inhibitor rifampicin.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Bacteriophage T4.; DNA -- Synthesis.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Genetics
Degree Grantor:
University of Arizona
Advisor:
Bernstein, Harris

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleCharacterization of T4 tertiary originsen_US
dc.creatorMenkens, Anne Elizabeth, 1958-en_US
dc.contributor.authorMenkens, Anne Elizabeth, 1958-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe bacteriophage T4 utilizes at least three modes of initiation of replication, termed primary, secondary and tertiary (Mosig, 1983; Kreuzer and Alberts, 1985). Two origins of replication have been isolated that utilize the tertiary mode of initiation. The DNA sequence requirements of the two tertiary origins have been characterized at the nucleotide level. Maximal replication of each origin-containing plasmid required both an intact gpmotA-dependent middle-mode promoter sequence and approximately 50 basepairs of the downstream region. In contrast, gpmotA-dependent transcription from the origin promoter was found to be independent of the downstream region. The requirement for a promoter element within the tertiary origins is striking, particularly since the replication of tertiary origin-containing plasmids is resistant to the RNA polymerase inhibitor rifampicin.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBacteriophage T4.en_US
dc.subjectDNA -- Synthesis.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineGeneticsen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBernstein, Harrisen_US
dc.identifier.proquest8902351en_US
dc.identifier.oclc22213368en_US
dc.identifier.bibrecord.b18416548en_US
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