Persistent Link:
http://hdl.handle.net/10150/282051
Title:
CHARACTERIZATION OF THE DOMINANT STATURE MUTANT OF MAIZE
Author:
Mastronardy, Joseph Francis
Issue Date:
1981
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
D8 originally designated as the dominant stature mutant of maize, was characterized and shown to be incompletely dominant. The study included morphological measurements, cytology, hormone studies, and enzyme and protein analysis. The effect of the D8 mutation can be detected after 40 hours of germination of the coleoptile. Dwarf (D8/d8) seedling length is 1/2 of the normal sib length for coleoptile, first leaf, and mesocotyl. The cell measurements indicate that cell elongation and cell division are involved in the size discrepancy. Mature dwarf plants have shorter internodes and the shorter, wider leaves are a darker green than the normal plant. The homozygous D8/D8 displays normal meiotic division and pollen formation is normal upto the 2 nucleate stage. Pollen viability of the homozygote is low and no seed was obtained in crosses involving this genotype. Several biological stains were used to test pollen viability with the results indicating greater than 85% viability for the heterozygote and less than 15.6% viability for the homozygote. The examination of the pachytene chromosomes of heterozygotes indicates a loop on a large chromosome. This loop is only found in the D8 heterozygote and implies a duplication or deficiency may be involved with the D8 phenotype. Avena straight growth bioassay for auxin displayed no significant difference in auxin production between dwarf and normal coleoptile tips. The D8 dwarf seedlings responded to the exogenous application of auxin, kinetin, and casamino acids in the same pattern as the normal seedlings, but never attained normal stature. Gibberellic acid (GA) and cyclic adenosine monophosphate (cAMP) exogenous applications displayed a difference in dwarf and normal response patterns and implies that the utilization or destruction of these substances may be involved. The investigation of Laemmli gel patterns for the three genotypes failed to show a difference. The soluble proteins formed 27 bands from the coleoptiles of each phenotype. Adh-1 gel patterns and pollen staining was utilized to examine the possibility of a deletion overlapping this locus. The Adh-1 locus has been mapped proximal to the D8 locus. The results indicate the Adh locus is not included in the putative D8 deletion.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Corn -- Morphology.; Corn -- Genetics.; Corn -- Physiology.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Genetics
Degree Grantor:
University of Arizona
Advisor:
Harris, Robert M.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleCHARACTERIZATION OF THE DOMINANT STATURE MUTANT OF MAIZEen_US
dc.creatorMastronardy, Joseph Francisen_US
dc.contributor.authorMastronardy, Joseph Francisen_US
dc.date.issued1981en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractD8 originally designated as the dominant stature mutant of maize, was characterized and shown to be incompletely dominant. The study included morphological measurements, cytology, hormone studies, and enzyme and protein analysis. The effect of the D8 mutation can be detected after 40 hours of germination of the coleoptile. Dwarf (D8/d8) seedling length is 1/2 of the normal sib length for coleoptile, first leaf, and mesocotyl. The cell measurements indicate that cell elongation and cell division are involved in the size discrepancy. Mature dwarf plants have shorter internodes and the shorter, wider leaves are a darker green than the normal plant. The homozygous D8/D8 displays normal meiotic division and pollen formation is normal upto the 2 nucleate stage. Pollen viability of the homozygote is low and no seed was obtained in crosses involving this genotype. Several biological stains were used to test pollen viability with the results indicating greater than 85% viability for the heterozygote and less than 15.6% viability for the homozygote. The examination of the pachytene chromosomes of heterozygotes indicates a loop on a large chromosome. This loop is only found in the D8 heterozygote and implies a duplication or deficiency may be involved with the D8 phenotype. Avena straight growth bioassay for auxin displayed no significant difference in auxin production between dwarf and normal coleoptile tips. The D8 dwarf seedlings responded to the exogenous application of auxin, kinetin, and casamino acids in the same pattern as the normal seedlings, but never attained normal stature. Gibberellic acid (GA) and cyclic adenosine monophosphate (cAMP) exogenous applications displayed a difference in dwarf and normal response patterns and implies that the utilization or destruction of these substances may be involved. The investigation of Laemmli gel patterns for the three genotypes failed to show a difference. The soluble proteins formed 27 bands from the coleoptiles of each phenotype. Adh-1 gel patterns and pollen staining was utilized to examine the possibility of a deletion overlapping this locus. The Adh-1 locus has been mapped proximal to the D8 locus. The results indicate the Adh locus is not included in the putative D8 deletion.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectCorn -- Morphology.en_US
dc.subjectCorn -- Genetics.en_US
dc.subjectCorn -- Physiology.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineGeneticsen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorHarris, Robert M.en_US
dc.identifier.proquest8205287en_US
dc.identifier.oclc8710769en_US
dc.identifier.bibrecord.b13917456en_US
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