ANALYSIS OF DNA RESTITUTION IN COTYLEDONARY TISSUE OF GOSSYPIUM BARBADENSE L. DURING GERMINATION

Persistent Link:
http://hdl.handle.net/10150/282042
Title:
ANALYSIS OF DNA RESTITUTION IN COTYLEDONARY TISSUE OF GOSSYPIUM BARBADENSE L. DURING GERMINATION
Author:
Shattuck, Vernon Irie
Issue Date:
1981
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
DNA repair in Gossypium barbadense L. cotyledonary tissue was examined in vivo during early germination. DNA strand breaks were initiated by applying ethyl methanesulfonate (EMS) during seed imbibition. Strand rejoining was monitored through a period of EMS induced DNS synthesis by means of velocity sedimentation analysis. There was an absence of DNA molecular weight increase as disclosed by the sedimentation profiles. It is presumed velocity sedimentation evaluation was insensitive for detecting in vivo restoration of cotyledonous DNA strand breaks. The altered DNA synthesis response perceived within treated seedling cotyledons perhaps reflects, but does not establish the operation of a cellular mutagenic repair system. EMS induced morphological mutant cotton plants arising during the course of this investigation were cytologically identified as possessing duplicate-deficient chromosomes.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
DNA repair.; Gossypium barbadense L.; Alkylation.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Plant Sciences
Degree Grantor:
University of Arizona
Advisor:
Katterman, Frank

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleANALYSIS OF DNA RESTITUTION IN COTYLEDONARY TISSUE OF GOSSYPIUM BARBADENSE L. DURING GERMINATIONen_US
dc.creatorShattuck, Vernon Irieen_US
dc.contributor.authorShattuck, Vernon Irieen_US
dc.date.issued1981en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractDNA repair in Gossypium barbadense L. cotyledonary tissue was examined in vivo during early germination. DNA strand breaks were initiated by applying ethyl methanesulfonate (EMS) during seed imbibition. Strand rejoining was monitored through a period of EMS induced DNS synthesis by means of velocity sedimentation analysis. There was an absence of DNA molecular weight increase as disclosed by the sedimentation profiles. It is presumed velocity sedimentation evaluation was insensitive for detecting in vivo restoration of cotyledonous DNA strand breaks. The altered DNA synthesis response perceived within treated seedling cotyledons perhaps reflects, but does not establish the operation of a cellular mutagenic repair system. EMS induced morphological mutant cotton plants arising during the course of this investigation were cytologically identified as possessing duplicate-deficient chromosomes.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectDNA repair.en_US
dc.subjectGossypium barbadense L.en_US
dc.subjectAlkylation.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorKatterman, Franken_US
dc.identifier.proquest8203733en_US
dc.identifier.oclc8706605en_US
dc.identifier.bibrecord.b13912768en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.