Stabilized supported lipid bilayers from polymerizable phospholipid monomers

Persistent Link:
http://hdl.handle.net/10150/280498
Title:
Stabilized supported lipid bilayers from polymerizable phospholipid monomers
Author:
Ross, Eric E.
Issue Date:
2003
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Lipid films are often described as potential surface coatings for the 'biofunctionalization' of solid interfaces because of the ability to support tethered and integrated receptor protein activity and their ability to suppress the non-specific adsorption of soluble proteins. One significant shortcoming of lipid assemblies is the inherent lack of stability required for many technological applications because the non-covalent forces between the constituent lipids are relatively weak. In this work, polymerized, supported lipid bilayers ((poly)PSLBs) composed of diene functionalized lipids have been prepared and characterized. Several parameters relating (poly)PSLB structure and stability to observations made in studies of polymerized bilayer vesicles will be described, including a comparison of UV photopolymerization and redox-initiated radical polymerization, the number and location of the polymerizable moieties in the lipid monomer, and a comparison to PSLBs produced with diacetylene lipids. Redox-initiated polymerization of films composed of bis-substituted diene lipids with at least one polymerizable moiety located near the acyl terminus produces dried PSLBs that are highly uniform and stable. All other conditions yielded PSLBs that contained a high density of defects after drying, including those formed from diacetylene lipids. The nonspecific adsorption of bovine serum albumin (BSA) is used to further characterize the polymer films to fluid PC bilayers, which have been established as protein adsorption "inert" surfaces. The results show that the protein resistance of a cross-linked (poly)PSLB composed of bis-sorbyl phosphatidylcholine (bis-SorbPC) is equivalent to that of a fluid PSLB composed of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), even after the former has been dried and rehydrated. Furthermore, the (poly)PSLB films can be formed in patterns or patterned with immobilized proteins by microprinting techniques which may facilitate their use in microarray detection schemes.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Chemistry, Analytical.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Chemistry
Degree Grantor:
University of Arizona
Advisor:
Saavedra, S. Scott

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleStabilized supported lipid bilayers from polymerizable phospholipid monomersen_US
dc.creatorRoss, Eric E.en_US
dc.contributor.authorRoss, Eric E.en_US
dc.date.issued2003en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractLipid films are often described as potential surface coatings for the 'biofunctionalization' of solid interfaces because of the ability to support tethered and integrated receptor protein activity and their ability to suppress the non-specific adsorption of soluble proteins. One significant shortcoming of lipid assemblies is the inherent lack of stability required for many technological applications because the non-covalent forces between the constituent lipids are relatively weak. In this work, polymerized, supported lipid bilayers ((poly)PSLBs) composed of diene functionalized lipids have been prepared and characterized. Several parameters relating (poly)PSLB structure and stability to observations made in studies of polymerized bilayer vesicles will be described, including a comparison of UV photopolymerization and redox-initiated radical polymerization, the number and location of the polymerizable moieties in the lipid monomer, and a comparison to PSLBs produced with diacetylene lipids. Redox-initiated polymerization of films composed of bis-substituted diene lipids with at least one polymerizable moiety located near the acyl terminus produces dried PSLBs that are highly uniform and stable. All other conditions yielded PSLBs that contained a high density of defects after drying, including those formed from diacetylene lipids. The nonspecific adsorption of bovine serum albumin (BSA) is used to further characterize the polymer films to fluid PC bilayers, which have been established as protein adsorption "inert" surfaces. The results show that the protein resistance of a cross-linked (poly)PSLB composed of bis-sorbyl phosphatidylcholine (bis-SorbPC) is equivalent to that of a fluid PSLB composed of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), even after the former has been dried and rehydrated. Furthermore, the (poly)PSLB films can be formed in patterns or patterned with immobilized proteins by microprinting techniques which may facilitate their use in microarray detection schemes.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectChemistry, Analytical.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorSaavedra, S. Scotten_US
dc.identifier.proquest3119980en_US
dc.identifier.bibrecord.b45645802en_US
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