Dynamic interactions between olfactory receptor axons and glial cells from the olfactory system of the moth Manduca sexta

Persistent Link:
http://hdl.handle.net/10150/280217
Title:
Dynamic interactions between olfactory receptor axons and glial cells from the olfactory system of the moth Manduca sexta
Author:
Tucker, Eric S
Issue Date:
2002
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Across species, glial cells in both peripheral and central nervous systems cooperate extensively with neurons to shape multiple aspects of neural development. In vertebrate and invertebrate olfactory systems, neuron-glia interactions are thought to underlie critical developmental events, including glomerulus formation, and the growth, sorting, and targeting of olfactory receptor neuron (ORN) axons. The olfactory system of the moth Manduca sexta has many similarities to vertebrate olfactory systems, and has been used extensively to explore intercellular interactions involved in the formation of the olfactory pathway. In particular, glial reduction experiments have implicated two types of central olfactory glia, the sorting zone and neuropil-associated glia, in axon sorting and glomerulus stabilization. The developmental roles of a third glial cell type, the antennal nerve glia, remain elusive, yet their peripheral origin and association with ORN axons are similar to mammalian olfactory ensheathing cells. The present body of work uses a defined co-culture system to characterize interactions between ORN axons growing from explants of olfactory receptor epithelium and glial cells from the primary olfactory system of Manduca. We have monitored how particular types of glia, known to influence the behavior of ORN axons in vivo, directly affect the behavior and morphology of individual ORN growth cones in vitro. Time-lapse imaging of neuron-glia cultures revealed that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone and neuropil-associated glial cells. In contrast, growth cones advance along the surfaces of antennal nerve glial cells without prolonged changes in morphology. Cytoskeletal staining of fixed preparations reinforced live-cell findings, as contact with sorting zone and neuropil-associated glial cells caused statistically significant changes in growth cone morphology. Finally, ORN axons induce antennal nerve glia, but not sorting zone or neuropil glia, to form multicellular arrays through proliferation and process extension. These findings have led to the formation of hypotheses concerning the nature of neuron-glia interactions in vivo.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Biology, Neuroscience.; Biology, Cell.; Biology, Animal Physiology.; Biology, Zoology.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Cell Biology and Anatomy
Degree Grantor:
University of Arizona
Advisor:
Tolbert, Leslie P.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleDynamic interactions between olfactory receptor axons and glial cells from the olfactory system of the moth Manduca sextaen_US
dc.creatorTucker, Eric Sen_US
dc.contributor.authorTucker, Eric Sen_US
dc.date.issued2002en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAcross species, glial cells in both peripheral and central nervous systems cooperate extensively with neurons to shape multiple aspects of neural development. In vertebrate and invertebrate olfactory systems, neuron-glia interactions are thought to underlie critical developmental events, including glomerulus formation, and the growth, sorting, and targeting of olfactory receptor neuron (ORN) axons. The olfactory system of the moth Manduca sexta has many similarities to vertebrate olfactory systems, and has been used extensively to explore intercellular interactions involved in the formation of the olfactory pathway. In particular, glial reduction experiments have implicated two types of central olfactory glia, the sorting zone and neuropil-associated glia, in axon sorting and glomerulus stabilization. The developmental roles of a third glial cell type, the antennal nerve glia, remain elusive, yet their peripheral origin and association with ORN axons are similar to mammalian olfactory ensheathing cells. The present body of work uses a defined co-culture system to characterize interactions between ORN axons growing from explants of olfactory receptor epithelium and glial cells from the primary olfactory system of Manduca. We have monitored how particular types of glia, known to influence the behavior of ORN axons in vivo, directly affect the behavior and morphology of individual ORN growth cones in vitro. Time-lapse imaging of neuron-glia cultures revealed that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone and neuropil-associated glial cells. In contrast, growth cones advance along the surfaces of antennal nerve glial cells without prolonged changes in morphology. Cytoskeletal staining of fixed preparations reinforced live-cell findings, as contact with sorting zone and neuropil-associated glial cells caused statistically significant changes in growth cone morphology. Finally, ORN axons induce antennal nerve glia, but not sorting zone or neuropil glia, to form multicellular arrays through proliferation and process extension. These findings have led to the formation of hypotheses concerning the nature of neuron-glia interactions in vivo.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBiology, Neuroscience.en_US
dc.subjectBiology, Cell.en_US
dc.subjectBiology, Animal Physiology.en_US
dc.subjectBiology, Zoology.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineCell Biology and Anatomyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorTolbert, Leslie P.en_US
dc.identifier.proquest3073269en_US
dc.identifier.bibrecord.b43478748en_US
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