The ARF-GEF ARNO and ARF6 regulate dendritic and axonal development in cultured rat hippocampal neurons

Persistent Link:
http://hdl.handle.net/10150/280184
Title:
The ARF-GEF ARNO and ARF6 regulate dendritic and axonal development in cultured rat hippocampal neurons
Author:
Hernandez Deviez, Delia Josefina
Issue Date:
2002
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The unique morphology of nerve cells is a key feature of the complex organization of the nervous system. Neurite extension and branching are dynamic processes that take place throughout the life of a neuron. The development of dendritic and axonal processes requires the coordination of membrane-cytoskeletal rearrangements to ensure the establishment of proper neural connections. These events are thought to be largely regulated by signaling pathways involving the Rho-family of small guanine triphosphatases (GTPases). Another family of small GTP-binding proteins, the ARF (ADP-ribosylation factor) family, has been implicated in the regulation of membrane transport and actin filament reorganization in non-neuronal cells. However, it is unknown what role ARF family members play in the modulation of these events during neurite extension and branching. Therefore, we have analyzed the role of the ARF-guanine exchange factor (GEF) ARNO (ARF-nucleotide binding site opener) in dendritic arbor development and axonal elongation and branching by overexpressing wild-type and mutant forms of ARNO and ARF6 in cultured rat hippocampal neurons. The overexpression of catalytically inactive ARNO and dominant-negative ARF6 result in increased dendritic branching as well as enhanced axonal extension and branching. Expression of inactive ARNO caused a redistribution of a subset of endosomes to the axonal plasma membrane and displacement of the profilin-binding protein Mena from the growth cone plasma membrane. These results suggest that ARNO through ARF6 negatively regulates dendritic branching and axonal elongation and branching during neural development.
Type:
text; Dissertation-Reproduction (electronic)
Keywords:
Biology, Neuroscience.; Biology, Cell.
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Cell Biology and Anatomy
Degree Grantor:
University of Arizona
Advisor:
Wilson, Jean M.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleThe ARF-GEF ARNO and ARF6 regulate dendritic and axonal development in cultured rat hippocampal neuronsen_US
dc.creatorHernandez Deviez, Delia Josefinaen_US
dc.contributor.authorHernandez Deviez, Delia Josefinaen_US
dc.date.issued2002en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe unique morphology of nerve cells is a key feature of the complex organization of the nervous system. Neurite extension and branching are dynamic processes that take place throughout the life of a neuron. The development of dendritic and axonal processes requires the coordination of membrane-cytoskeletal rearrangements to ensure the establishment of proper neural connections. These events are thought to be largely regulated by signaling pathways involving the Rho-family of small guanine triphosphatases (GTPases). Another family of small GTP-binding proteins, the ARF (ADP-ribosylation factor) family, has been implicated in the regulation of membrane transport and actin filament reorganization in non-neuronal cells. However, it is unknown what role ARF family members play in the modulation of these events during neurite extension and branching. Therefore, we have analyzed the role of the ARF-guanine exchange factor (GEF) ARNO (ARF-nucleotide binding site opener) in dendritic arbor development and axonal elongation and branching by overexpressing wild-type and mutant forms of ARNO and ARF6 in cultured rat hippocampal neurons. The overexpression of catalytically inactive ARNO and dominant-negative ARF6 result in increased dendritic branching as well as enhanced axonal extension and branching. Expression of inactive ARNO caused a redistribution of a subset of endosomes to the axonal plasma membrane and displacement of the profilin-binding protein Mena from the growth cone plasma membrane. These results suggest that ARNO through ARF6 negatively regulates dendritic branching and axonal elongation and branching during neural development.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.subjectBiology, Neuroscience.en_US
dc.subjectBiology, Cell.en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineCell Biology and Anatomyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWilson, Jean M.en_US
dc.identifier.proquest3073231en_US
dc.identifier.bibrecord.b4347195xen_US
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