Isolation and characterization of high copy number suppressors of the SOS system in Escherichia coli

Persistent Link:
http://hdl.handle.net/10150/278390
Title:
Isolation and characterization of high copy number suppressors of the SOS system in Escherichia coli
Author:
Hunter, Rosalyn Alexandria, 1966-
Issue Date:
1993
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In an attempt to find new genes that are involved in the induction of the SOS system of Escherichia coli, a plasmid library of Escherichia coli K-12 DNA cut with EcoRI was created in a pUC plasmid. The plasmids were transformed into the Escherichia coli strain AT492 containing a sulA::lacZ fusion. Colonies which did not show lacZ expression when the SOS systems had been induced by Mitomycin C were isolated. Four plasmids were found to suppress SOS induction when they were highly expressed. Kohara phage hybridization and restriction mapping suggest that these plasmids contain the genes for lexA and a truncated recA gene ending at the EcoRI site at basepair 1016.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Biology, Cell.; Biology, Microbiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Molecular and cellular biology
Degree Grantor:
University of Arizona
Advisor:
Mount, David

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleIsolation and characterization of high copy number suppressors of the SOS system in Escherichia colien_US
dc.creatorHunter, Rosalyn Alexandria, 1966-en_US
dc.contributor.authorHunter, Rosalyn Alexandria, 1966-en_US
dc.date.issued1993en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn an attempt to find new genes that are involved in the induction of the SOS system of Escherichia coli, a plasmid library of Escherichia coli K-12 DNA cut with EcoRI was created in a pUC plasmid. The plasmids were transformed into the Escherichia coli strain AT492 containing a sulA::lacZ fusion. Colonies which did not show lacZ expression when the SOS systems had been induced by Mitomycin C were isolated. Four plasmids were found to suppress SOS induction when they were highly expressed. Kohara phage hybridization and restriction mapping suggest that these plasmids contain the genes for lexA and a truncated recA gene ending at the EcoRI site at basepair 1016.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectBiology, Cell.en_US
dc.subjectBiology, Microbiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMolecular and cellular biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorMount, Daviden_US
dc.identifier.proquest1356814en_US
dc.identifier.bibrecord.b31469553en_US
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