Use of staphylococcal enterotoxin A-induced interleukin 2 production as an indicator of immunotoxicity

Persistent Link:
http://hdl.handle.net/10150/278371
Title:
Use of staphylococcal enterotoxin A-induced interleukin 2 production as an indicator of immunotoxicity
Author:
Reid, Lynnda Louise, 1949-
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
An in vitro assay measuring IL-2 production as an index of compromised immune function was developed using primary splenocytes from female C57BL/6 mice pretreated with olive oil, 30 mg/kg Cyclosporin A, or 220-880 mg/kg benzene. Splenocytes were mitogen-stimulated to produce IL-2 with SEA, Con A or PHA. Benzene induced inhibition of IL-2 production was demonstrated by incubating the splenocyte culture supernatant fraction with an IL-2 dependent T-lymphocytic cell line. Cell proliferation was measured by blastogenesis and MTT reduction as an index of IL-2 mediated response. Benzene and cyclosporin dosed animals demonstrated a significant decrease in splenocyte proliferation. However, cell proliferation in IL-2 dependent cells was inhibited only with the higher doses of benzene indicative of a dose dependent IL- 2 mediated suppression. Chemically induced impairment of IL-2 mediated immune function may be detected with this assay.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Health Sciences, Pharmacology.; Health Sciences, Immunology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Gandolfi, A. Jay

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleUse of staphylococcal enterotoxin A-induced interleukin 2 production as an indicator of immunotoxicityen_US
dc.creatorReid, Lynnda Louise, 1949-en_US
dc.contributor.authorReid, Lynnda Louise, 1949-en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAn in vitro assay measuring IL-2 production as an index of compromised immune function was developed using primary splenocytes from female C57BL/6 mice pretreated with olive oil, 30 mg/kg Cyclosporin A, or 220-880 mg/kg benzene. Splenocytes were mitogen-stimulated to produce IL-2 with SEA, Con A or PHA. Benzene induced inhibition of IL-2 production was demonstrated by incubating the splenocyte culture supernatant fraction with an IL-2 dependent T-lymphocytic cell line. Cell proliferation was measured by blastogenesis and MTT reduction as an index of IL-2 mediated response. Benzene and cyclosporin dosed animals demonstrated a significant decrease in splenocyte proliferation. However, cell proliferation in IL-2 dependent cells was inhibited only with the higher doses of benzene indicative of a dose dependent IL- 2 mediated suppression. Chemically induced impairment of IL-2 mediated immune function may be detected with this assay.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectHealth Sciences, Pharmacology.en_US
dc.subjectHealth Sciences, Immunology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorGandolfi, A. Jayen_US
dc.identifier.proquest1341285en_US
dc.identifier.bibrecord.b26341608en_US
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