In vitro filament formation by the major sperm protein (MSP) of Caenorhabditis elegans

Persistent Link:
http://hdl.handle.net/10150/278319
Title:
In vitro filament formation by the major sperm protein (MSP) of Caenorhabditis elegans
Author:
Smith, Michael Joseph, 1968-
Issue Date:
1993
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Nematode sperm are asymmetric cells which exhibit an amoeboid motility similar to that seen in other actin-rich eukaryotic cells. However, the pseudopod of these cells is not packed with actin, but with 2-5 nm fine filaments of major sperm protein (MSP), a family of small, basic sperm-specific polypeptides which constitute ∼15% of the total cellular protein of the sperm. In Caenorhabditis elegans, the MSPs are encoded by a large multigene family of ∼60 members which has made genetic analysis of MSP and its role in cell motility impossible. In this work, C. elegans pMSP 56 has been synthesized at high levels using an E. coli expression vector. Two methods of pMSP 56 purification have been developed, one resulting in 60-70% pure MSP and the second resulting in 99% pure MSP. Both of these purified proteins are also capable of assembling into 2.5 ± 0.4 nm diameter filaments in the presence of 30% ethanol. These filaments seem to have a definite substructure, although detailed analysis of the substructure has not been carried out. The ability to produce large amounts of pure MSP and its ability to assemble into filaments represents a first step in developing an in vitro assay system for the MSP cytoskeleton. (Abstract shortened by UMI.)
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Biology, Cell.; Chemistry, Biochemistry.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Ward, Samuel

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleIn vitro filament formation by the major sperm protein (MSP) of Caenorhabditis elegansen_US
dc.creatorSmith, Michael Joseph, 1968-en_US
dc.contributor.authorSmith, Michael Joseph, 1968-en_US
dc.date.issued1993en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNematode sperm are asymmetric cells which exhibit an amoeboid motility similar to that seen in other actin-rich eukaryotic cells. However, the pseudopod of these cells is not packed with actin, but with 2-5 nm fine filaments of major sperm protein (MSP), a family of small, basic sperm-specific polypeptides which constitute ∼15% of the total cellular protein of the sperm. In Caenorhabditis elegans, the MSPs are encoded by a large multigene family of ∼60 members which has made genetic analysis of MSP and its role in cell motility impossible. In this work, C. elegans pMSP 56 has been synthesized at high levels using an E. coli expression vector. Two methods of pMSP 56 purification have been developed, one resulting in 60-70% pure MSP and the second resulting in 99% pure MSP. Both of these purified proteins are also capable of assembling into 2.5 ± 0.4 nm diameter filaments in the presence of 30% ethanol. These filaments seem to have a definite substructure, although detailed analysis of the substructure has not been carried out. The ability to produce large amounts of pure MSP and its ability to assemble into filaments represents a first step in developing an in vitro assay system for the MSP cytoskeleton. (Abstract shortened by UMI.)en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectBiology, Cell.en_US
dc.subjectChemistry, Biochemistry.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWard, Samuelen_US
dc.identifier.proquest1352391en_US
dc.identifier.bibrecord.b27056818en_US
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