Application of recombinant techniques for detection of the penaeid shrimp baculovirus, BP

Persistent Link:
http://hdl.handle.net/10150/278301
Title:
Application of recombinant techniques for detection of the penaeid shrimp baculovirus, BP
Author:
Bruce, Linda Diane, 1968-
Issue Date:
1993
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Research was conducted to develop gene probes to Baculovirus penaei (BP) from a cloning of BP genomic DNA and to test the applications of the gene probes. Five non-homologous probes representing >20% of the total BP genome were tested in dot blot and in situ hybridization assays. Four different dot blot hybridization assay systems were evaluated. Results obtained from radioactive labeling of the probes indicate that the probes are specific for BP DNA, while the results obtained from the three non-radioactive labeling systems suggest problems with non-specific reactivity and sample preparation for these methods. The results also indicated that in situ hybridization is presently the optimum assaying system for BP gene probes. The BP gene probes were used with an in situ hybridization assay to gather initial data concerning the mode of transmission for BP.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Microbiology.; Biology, Veterinary Science.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Lightner, Donald V.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleApplication of recombinant techniques for detection of the penaeid shrimp baculovirus, BPen_US
dc.creatorBruce, Linda Diane, 1968-en_US
dc.contributor.authorBruce, Linda Diane, 1968-en_US
dc.date.issued1993en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractResearch was conducted to develop gene probes to Baculovirus penaei (BP) from a cloning of BP genomic DNA and to test the applications of the gene probes. Five non-homologous probes representing >20% of the total BP genome were tested in dot blot and in situ hybridization assays. Four different dot blot hybridization assay systems were evaluated. Results obtained from radioactive labeling of the probes indicate that the probes are specific for BP DNA, while the results obtained from the three non-radioactive labeling systems suggest problems with non-specific reactivity and sample preparation for these methods. The results also indicated that in situ hybridization is presently the optimum assaying system for BP gene probes. The BP gene probes were used with an in situ hybridization assay to gather initial data concerning the mode of transmission for BP.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Microbiology.en_US
dc.subjectBiology, Veterinary Science.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLightner, Donald V.en_US
dc.identifier.proquest1352361en_US
dc.identifier.bibrecord.b27051705en_US
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