Occurrence of enteric viruses in disposable diapers from three landfills

Persistent Link:
http://hdl.handle.net/10150/278256
Title:
Occurrence of enteric viruses in disposable diapers from three landfills
Author:
Huber, Mary Susan, 1961-
Issue Date:
1992
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Disposable diapers are a possible source of infectious enteric viruses that are disposed of in landfills. A total of 210 disposable diapers were collected from seven sites and ten depths at three landfills. Of these total, 110 diapers were processed with a 1.5% beef-extract-elution organic-flocculation-concentration method to recover viruses. The concentrated samples were assayed on BGM cell cultures for the detection of enteroviruses and with cDNA probes specific for poliovirus, hepatitis A virus and rotavirus. Enteroviruses were not detected in any sample assayed using cell culture techniques. Three samples were positive using nucleic acid probes for poliovirus. These results suggest that poliovirus RNA was present in some diapers but that the viruses were not viable after two years or longer in a landfill.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Microbiology.; Engineering, Sanitary and Municipal.; Environmental Sciences.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Gerba, Charles P.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleOccurrence of enteric viruses in disposable diapers from three landfillsen_US
dc.creatorHuber, Mary Susan, 1961-en_US
dc.contributor.authorHuber, Mary Susan, 1961-en_US
dc.date.issued1992en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractDisposable diapers are a possible source of infectious enteric viruses that are disposed of in landfills. A total of 210 disposable diapers were collected from seven sites and ten depths at three landfills. Of these total, 110 diapers were processed with a 1.5% beef-extract-elution organic-flocculation-concentration method to recover viruses. The concentrated samples were assayed on BGM cell cultures for the detection of enteroviruses and with cDNA probes specific for poliovirus, hepatitis A virus and rotavirus. Enteroviruses were not detected in any sample assayed using cell culture techniques. Three samples were positive using nucleic acid probes for poliovirus. These results suggest that poliovirus RNA was present in some diapers but that the viruses were not viable after two years or longer in a landfill.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Microbiology.en_US
dc.subjectEngineering, Sanitary and Municipal.en_US
dc.subjectEnvironmental Sciences.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorGerba, Charles P.en_US
dc.identifier.proquest1351345en_US
dc.identifier.bibrecord.b27149328en_US
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