A comparison of neutrophil-coronary endothelial cell adhesion under static and flow conditions

Persistent Link:
http://hdl.handle.net/10150/278179
Title:
A comparison of neutrophil-coronary endothelial cell adhesion under static and flow conditions
Author:
Stringer, Steven Kennith, 1955-
Issue Date:
1992
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Neutrophils have been implicated in exacerbating ischemia/reperfusion injury in hearts. This study was undertaken to compare the adhesion of isolated rabbit neutrophils to rabbit coronary endothelial cells grown in culture under static and flow conditions. Also, the effects of a platelet activating factor (PAF) antagonist (SDZ 64-412) were evaluated in both systems along with monoclonal antibodies (mab) against specific adhesion proteins in the static system. Comparable concentration response curves for SDZ 64-412 were obtained in both systems with a greater decrease in adhesion seen in the flow system versus the static system. Both systems showed a significant inhibition of adhesion with increased concentration. The mabs against CD11a, CD11b, CD18, and ICAM reduced adhesion to 94.8%, 76.5%, 72.6%, and 26.7%, respectively. In conclusion, neutrophil/coronary endothelial cell adhesion can be quantified in both static and flow systems. Also, SDZ 64-412 effectively inhibits the adhesion of these two cell types to an even greater extent than certain adhesion protein mabs.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Health Sciences, Pharmacology.; Health Sciences, Medicine and Surgery.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Larson, Douglas F.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleA comparison of neutrophil-coronary endothelial cell adhesion under static and flow conditionsen_US
dc.creatorStringer, Steven Kennith, 1955-en_US
dc.contributor.authorStringer, Steven Kennith, 1955-en_US
dc.date.issued1992en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNeutrophils have been implicated in exacerbating ischemia/reperfusion injury in hearts. This study was undertaken to compare the adhesion of isolated rabbit neutrophils to rabbit coronary endothelial cells grown in culture under static and flow conditions. Also, the effects of a platelet activating factor (PAF) antagonist (SDZ 64-412) were evaluated in both systems along with monoclonal antibodies (mab) against specific adhesion proteins in the static system. Comparable concentration response curves for SDZ 64-412 were obtained in both systems with a greater decrease in adhesion seen in the flow system versus the static system. Both systems showed a significant inhibition of adhesion with increased concentration. The mabs against CD11a, CD11b, CD18, and ICAM reduced adhesion to 94.8%, 76.5%, 72.6%, and 26.7%, respectively. In conclusion, neutrophil/coronary endothelial cell adhesion can be quantified in both static and flow systems. Also, SDZ 64-412 effectively inhibits the adhesion of these two cell types to an even greater extent than certain adhesion protein mabs.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectHealth Sciences, Pharmacology.en_US
dc.subjectHealth Sciences, Medicine and Surgery.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLarson, Douglas F.en_US
dc.identifier.proquest1349465en_US
dc.identifier.bibrecord.b27693016en_US
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