Characterization of aneuploids in pyrethrum, (Chrysanthemum cinerariaefolium), by cytology, morphology, and flow cytometry

Persistent Link:
http://hdl.handle.net/10150/278167
Title:
Characterization of aneuploids in pyrethrum, (Chrysanthemum cinerariaefolium), by cytology, morphology, and flow cytometry
Author:
Forkan, Kathryn Marie, 1958-
Issue Date:
1992
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Aneuploid plants are often used to locate genes or establish linkage, but first, they must be available. In Chrysanthemum cinerariaefolium Vis., a plant that produces pyrethrin, a relatively safe natural insecticide, aneuploids had not been categorized before. This research proposed to characterize aneuploids by morphology, cytology, nuclear DNA content, and pyrethrin composition. Aneuploid progeny of triploid parents were examined cytologically and morphologically. HPLC was used to establish pyrethrin composition and nuclear DNA content was calculated from flow cytometry. Five distinct phenotypes were found that may indicate aneuploid status, but not exact chromosome number. Exact chromosome number could only be distinguished cytologically, and ranged from 17 to 36, (2n = 18 for the diploid). Three trisomics were located. Nuclear DNA content indicated diploid or triploid status but not aneuploidy. Some of the aneuploids categorized may be useful in further research to locate pyrethrin genes or their linkages.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Genetics.; Biology, Botany.; Biology, Genetics.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleCharacterization of aneuploids in pyrethrum, (Chrysanthemum cinerariaefolium), by cytology, morphology, and flow cytometryen_US
dc.creatorForkan, Kathryn Marie, 1958-en_US
dc.contributor.authorForkan, Kathryn Marie, 1958-en_US
dc.date.issued1992en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAneuploid plants are often used to locate genes or establish linkage, but first, they must be available. In Chrysanthemum cinerariaefolium Vis., a plant that produces pyrethrin, a relatively safe natural insecticide, aneuploids had not been categorized before. This research proposed to characterize aneuploids by morphology, cytology, nuclear DNA content, and pyrethrin composition. Aneuploid progeny of triploid parents were examined cytologically and morphologically. HPLC was used to establish pyrethrin composition and nuclear DNA content was calculated from flow cytometry. Five distinct phenotypes were found that may indicate aneuploid status, but not exact chromosome number. Exact chromosome number could only be distinguished cytologically, and ranged from 17 to 36, (2n = 18 for the diploid). Three trisomics were located. Nuclear DNA content indicated diploid or triploid status but not aneuploidy. Some of the aneuploids categorized may be useful in further research to locate pyrethrin genes or their linkages.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Genetics.en_US
dc.subjectBiology, Botany.en_US
dc.subjectBiology, Genetics.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1349140en_US
dc.identifier.bibrecord.b27657164en_US
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