Macrofiber properties of the Bacillus subtilis strain carrying a transposon insertion

Persistent Link:
http://hdl.handle.net/10150/278127
Title:
Macrofiber properties of the Bacillus subtilis strain carrying a transposon insertion
Author:
Su, Ling-Ing, 1961-
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Recently, a new strain of B. subtilis (Bull913) carrying Tn917, a Streptococcus-derived transposon that confers erythromycin and lincomycin resistance, in the host chromosome has been isolated that grows as multistrand helical structures. Bull913 can produce macrofibers whose properties are like another B. subtilis strain FJ7. FJ7 is a lyt-2 mutant produced by chemical mutagenesis which can form macrofibers with twists ranging from right to left handed. Bull913 also has this spectrum of twisted states. Bull913 macrofibers are similar to FJ7 but are longer lived than FJ7. The Macrofiber properties of Bull913 were compared to FJ7 using growth in liquid media supplemented with (NH₄)₂SO₄ or MgSO₄ -- conditions giving LH or RH macrofibers, respectively for FJ7. The autolysin activities and cell walls substrate properties of Bull913 were biochemically analyzed and compared to those of strains 168 and FJ7. Polyacrylamide gel electrophoresis (PAGE) was used to compare the wall proteins from all three strains. Finally, genetic studies of Bull913 were carried out to determine if the defect in Bull913 is in the lyt-1, lyt-2 or lyt-15 genes, or in a new gene. Both biochemical analysis and genetic studies suggest that Bul1913 carries Tn917 in the lyt-2 gene.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Microbiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleMacrofiber properties of the Bacillus subtilis strain carrying a transposon insertionen_US
dc.creatorSu, Ling-Ing, 1961-en_US
dc.contributor.authorSu, Ling-Ing, 1961-en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractRecently, a new strain of B. subtilis (Bull913) carrying Tn917, a Streptococcus-derived transposon that confers erythromycin and lincomycin resistance, in the host chromosome has been isolated that grows as multistrand helical structures. Bull913 can produce macrofibers whose properties are like another B. subtilis strain FJ7. FJ7 is a lyt-2 mutant produced by chemical mutagenesis which can form macrofibers with twists ranging from right to left handed. Bull913 also has this spectrum of twisted states. Bull913 macrofibers are similar to FJ7 but are longer lived than FJ7. The Macrofiber properties of Bull913 were compared to FJ7 using growth in liquid media supplemented with (NH₄)₂SO₄ or MgSO₄ -- conditions giving LH or RH macrofibers, respectively for FJ7. The autolysin activities and cell walls substrate properties of Bull913 were biochemically analyzed and compared to those of strains 168 and FJ7. Polyacrylamide gel electrophoresis (PAGE) was used to compare the wall proteins from all three strains. Finally, genetic studies of Bull913 were carried out to determine if the defect in Bull913 is in the lyt-1, lyt-2 or lyt-15 genes, or in a new gene. Both biochemical analysis and genetic studies suggest that Bul1913 carries Tn917 in the lyt-2 gene.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Microbiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1341837en_US
dc.identifier.bibrecord.b26429895en_US
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