Development of procedures towards the somatic hybridization of alfalfa and Medicago marina L.

Persistent Link:
http://hdl.handle.net/10150/278034
Title:
Development of procedures towards the somatic hybridization of alfalfa and Medicago marina L.
Author:
Hocker, Anna Margaret, 1960-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Protoplasts were isolated from mesophyll tissue, callus and seedling cotyledons of Medicago sativa L. cv. Regen S and the halophyte M. marina L. Cotyledon protoplasts of Regen S were cultured in protoplast and cell culture media used previously for alfalfa protoplast culture and in media that had been simplified. There were no differences in the plating efficiencies of protoplasts cultured in the simple and complex media, but cells produced in the latter were greener and they colonized sooner. Protoplasts of M. marina grew at one-half the rate of Regen S protoplasts. Etiolated cotyledon protoplasts of Regen S were fused at 31°C using a solution containing PEG, DMSO and calcium at high pH. The frequency of fusion was 16% of the surviving protoplasts. These methods for protoplast isolation, culture and fusion should be useful in the somatic hybridization of alfalfa and M. marina.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Agriculture, Agronomy.; Biology, General.; Agriculture, Plant Culture.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Katterman, Frank R. H.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleDevelopment of procedures towards the somatic hybridization of alfalfa and Medicago marina L.en_US
dc.creatorHocker, Anna Margaret, 1960-en_US
dc.contributor.authorHocker, Anna Margaret, 1960-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractProtoplasts were isolated from mesophyll tissue, callus and seedling cotyledons of Medicago sativa L. cv. Regen S and the halophyte M. marina L. Cotyledon protoplasts of Regen S were cultured in protoplast and cell culture media used previously for alfalfa protoplast culture and in media that had been simplified. There were no differences in the plating efficiencies of protoplasts cultured in the simple and complex media, but cells produced in the latter were greener and they colonized sooner. Protoplasts of M. marina grew at one-half the rate of Regen S protoplasts. Etiolated cotyledon protoplasts of Regen S were fused at 31°C using a solution containing PEG, DMSO and calcium at high pH. The frequency of fusion was 16% of the surviving protoplasts. These methods for protoplast isolation, culture and fusion should be useful in the somatic hybridization of alfalfa and M. marina.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectAgriculture, Agronomy.en_US
dc.subjectBiology, General.en_US
dc.subjectAgriculture, Plant Culture.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorKatterman, Frank R. H.en_US
dc.identifier.proquest1346698en_US
dc.identifier.bibrecord.b27251895en_US
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