Characterization of new iodinated peptide radioligand for delta opioid receptors

Persistent Link:
http://hdl.handle.net/10150/278021
Title:
Characterization of new iodinated peptide radioligand for delta opioid receptors
Author:
Fang, Lei, 1966-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
New radioligand [4'-¹²⁵I-[Phe⁴]DPDPE ([¹²⁵I] DPDPE) was prepared from [4'-NH₂-Phe⁴] DPDPE. Its binding characteristics were determined using rat brain membranes. Saturation studies showed one binding site with K(d) = 421 ± 67 pM and B(max) = 36.4 ± 2.7 fmol/mg protein. Kinetic studies showed association rate constant k₊₁ = 5.80 ± 0.88 x 10⁷ M⁻¹ min⁻¹ and dissociation rate constant k-1 = 0.917 ± 0.117 x 10⁻² min⁻¹. Competitive inhibition studies showed high selectivity for delta opioid receptors. Distribution of [¹²⁵I] DPDPE labeled sites in rat brain (autoradiography) is consistent with previous results using other radioligands. The inhibition constants of [¹²⁵I] DPDPE binding by delta selective ligands were similar in mouse brain and mouse vas deferens, and no significantly different values were determined in rat brain. These data indicate that [¹²⁵I] DPDPE due to high specific activity is valuable for characterization of delta opioid receptors.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Health Sciences, Pharmacology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Yamamura, Henry I.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleCharacterization of new iodinated peptide radioligand for delta opioid receptorsen_US
dc.creatorFang, Lei, 1966-en_US
dc.contributor.authorFang, Lei, 1966-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNew radioligand [4'-¹²⁵I-[Phe⁴]DPDPE ([¹²⁵I] DPDPE) was prepared from [4'-NH₂-Phe⁴] DPDPE. Its binding characteristics were determined using rat brain membranes. Saturation studies showed one binding site with K(d) = 421 ± 67 pM and B(max) = 36.4 ± 2.7 fmol/mg protein. Kinetic studies showed association rate constant k₊₁ = 5.80 ± 0.88 x 10⁷ M⁻¹ min⁻¹ and dissociation rate constant k-1 = 0.917 ± 0.117 x 10⁻² min⁻¹. Competitive inhibition studies showed high selectivity for delta opioid receptors. Distribution of [¹²⁵I] DPDPE labeled sites in rat brain (autoradiography) is consistent with previous results using other radioligands. The inhibition constants of [¹²⁵I] DPDPE binding by delta selective ligands were similar in mouse brain and mouse vas deferens, and no significantly different values were determined in rat brain. These data indicate that [¹²⁵I] DPDPE due to high specific activity is valuable for characterization of delta opioid receptors.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectHealth Sciences, Pharmacology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorYamamura, Henry I.en_US
dc.identifier.proquest1346680en_US
dc.identifier.bibrecord.b27251512en_US
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