Methods development for the in vitro assessment of cutaneous drug metabolism

Persistent Link:
http://hdl.handle.net/10150/277962
Title:
Methods development for the in vitro assessment of cutaneous drug metabolism
Author:
Thohan, Sanjeev, 1962-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The major objective of this work was development of procedures for the quantification of cutaneous microsomal cytochrome P-450 mediated metabolism using model substrates. The Fischer-344 rat was the species of choice for this developmental work. The versatility of this method was demonstrated through its application to a variety of species including man. An extension of the rat microsomal work entailed the use of inducing agents, specifically, the Aroclor series of polychlorinated biphenyls. The effects of these inducing agents were demonstrated and characterized in skin and liver with respect to microsomal marker enzymes and substrate metabolism. In addition to the microsomal studies, the use of rat skin explants in culture, provided a simple system for the characterization of phase I and phase II coupled metabolism of the model substrates.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Health Sciences, Pharmacology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Sipes, I. Glenn

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleMethods development for the in vitro assessment of cutaneous drug metabolismen_US
dc.creatorThohan, Sanjeev, 1962-en_US
dc.contributor.authorThohan, Sanjeev, 1962-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe major objective of this work was development of procedures for the quantification of cutaneous microsomal cytochrome P-450 mediated metabolism using model substrates. The Fischer-344 rat was the species of choice for this developmental work. The versatility of this method was demonstrated through its application to a variety of species including man. An extension of the rat microsomal work entailed the use of inducing agents, specifically, the Aroclor series of polychlorinated biphenyls. The effects of these inducing agents were demonstrated and characterized in skin and liver with respect to microsomal marker enzymes and substrate metabolism. In addition to the microsomal studies, the use of rat skin explants in culture, provided a simple system for the characterization of phase I and phase II coupled metabolism of the model substrates.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectHealth Sciences, Pharmacology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorSipes, I. Glennen_US
dc.identifier.proquest1345441en_US
dc.identifier.bibrecord.b27031184en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.