Theoretical and experimental studies of the plasma protein binding of high affinity binding drugs

Persistent Link:
http://hdl.handle.net/10150/277955
Title:
Theoretical and experimental studies of the plasma protein binding of high affinity binding drugs
Author:
Lee, Hui-Chih, 1963-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
A disadvantage of traditional equilibrium dialysis for highly protein bound drugs is the analytically low drug concentration found on the buffer side. We propose to replace a certain percentage of buffer with plasma containing drug in order to increase the total drug concentration on the buffer side. Computer simulations were performed to examine the effects of the percentage of plasma replacement of the buffer upon the increase of the total drug concentration on the buffer side after equilibrium dialysis. Further simulation results indicated that the development of a concise equation estimating the drug's equilibrium association binding constant (Ka) was feasible. Two high binding drugs, diazepam and nortriptyline, were examined to verify the advantages offered by the new proposed method and their Ka values were computed using the experimental results and the mathematical equation developed. The resulting data agreed well with theoretical predictions.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Chemistry, Pharmaceutical.; Health Sciences, Pharmacy.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Mayersohn, Michael

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleTheoretical and experimental studies of the plasma protein binding of high affinity binding drugsen_US
dc.creatorLee, Hui-Chih, 1963-en_US
dc.contributor.authorLee, Hui-Chih, 1963-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractA disadvantage of traditional equilibrium dialysis for highly protein bound drugs is the analytically low drug concentration found on the buffer side. We propose to replace a certain percentage of buffer with plasma containing drug in order to increase the total drug concentration on the buffer side. Computer simulations were performed to examine the effects of the percentage of plasma replacement of the buffer upon the increase of the total drug concentration on the buffer side after equilibrium dialysis. Further simulation results indicated that the development of a concise equation estimating the drug's equilibrium association binding constant (Ka) was feasible. Two high binding drugs, diazepam and nortriptyline, were examined to verify the advantages offered by the new proposed method and their Ka values were computed using the experimental results and the mathematical equation developed. The resulting data agreed well with theoretical predictions.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectChemistry, Pharmaceutical.en_US
dc.subjectHealth Sciences, Pharmacy.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorMayersohn, Michaelen_US
dc.identifier.proquest1345433en_US
dc.identifier.bibrecord.b27031093en_US
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