Expression of a mammalian cytochrome P-450 in Nicotiana tabacum for bioremediation of PCB contaminated soils

Persistent Link:
http://hdl.handle.net/10150/277929
Title:
Expression of a mammalian cytochrome P-450 in Nicotiana tabacum for bioremediation of PCB contaminated soils
Author:
Wall, Victor Daniel, III, 1963-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Polychlorinated biphenyls (PCBs) are resistant to metabolism in most animal species. The dog has the unique ability to metabolize and eliminate certain PCB congeners, as a result of the activity of the cytochrome P450 isozyme PBD-2. An expressible cDNA coding for PBD-2 has been introduced into the genome of tobacco plants. The PBD-2 cDNA coding sequence and a screenable marker gene coding for neomycin phosphotransferase II were introduced into tobacco leaf disks using a binary Agrobacterium tumefaciens vector system. Southern and Western blot analysis have confirmed chromosomal integration of the cDNA and expression of the PBD-2 polypeptide. Differential centrifugation and Western blot analyses have shown the PBD-2 protein to be associated with a membrane fraction in transgenic tobacco leaf homogenates. Measurements of marker enzymes from linear sucrose gradient fractions and Western blotting show the PBD-2 protein to be associated with the endoplasmic reticulum. Our goal is to develop transgenic plants in which the PBD-2 protein metabolizes PCBs, thus providing a novel method for bioremediation of PCB-contaminated soils.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Health Sciences, Toxicology.; Environmental Sciences.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Halpert, J. R.; Bourque, D. P.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleExpression of a mammalian cytochrome P-450 in Nicotiana tabacum for bioremediation of PCB contaminated soilsen_US
dc.creatorWall, Victor Daniel, III, 1963-en_US
dc.contributor.authorWall, Victor Daniel, III, 1963-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractPolychlorinated biphenyls (PCBs) are resistant to metabolism in most animal species. The dog has the unique ability to metabolize and eliminate certain PCB congeners, as a result of the activity of the cytochrome P450 isozyme PBD-2. An expressible cDNA coding for PBD-2 has been introduced into the genome of tobacco plants. The PBD-2 cDNA coding sequence and a screenable marker gene coding for neomycin phosphotransferase II were introduced into tobacco leaf disks using a binary Agrobacterium tumefaciens vector system. Southern and Western blot analysis have confirmed chromosomal integration of the cDNA and expression of the PBD-2 polypeptide. Differential centrifugation and Western blot analyses have shown the PBD-2 protein to be associated with a membrane fraction in transgenic tobacco leaf homogenates. Measurements of marker enzymes from linear sucrose gradient fractions and Western blotting show the PBD-2 protein to be associated with the endoplasmic reticulum. Our goal is to develop transgenic plants in which the PBD-2 protein metabolizes PCBs, thus providing a novel method for bioremediation of PCB-contaminated soils.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectHealth Sciences, Toxicology.en_US
dc.subjectEnvironmental Sciences.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorHalpert, J. R.en_US
dc.contributor.advisorBourque, D. P.en_US
dc.identifier.proquest1345385en_US
dc.identifier.bibrecord.b2700353xen_US
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