Biodegradation of aromatic compounds in the presence of secondary substrates

Persistent Link:
http://hdl.handle.net/10150/277887
Title:
Biodegradation of aromatic compounds in the presence of secondary substrates
Author:
Vogel, Catherine, 1959-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Experiments were conducted to examine the biodegradability of three aromatic compounds typically found at groundwater contamination sites; benzene, toluene, and chlorobenzene. A pure Pseudomonas species, JS6, was used in all batch experiments. JS6 was grown on benzene, chlorobenzene, toluene, yeast extract, or glucose as sole source of carbon and energy. These cultures were in turn used to test the biodegradability of the three aromatics of interest both in the presence and absence of the chemical used for acclimation. The results indicated that the presence of a non-aromatic substrate (yeast extract or glucose) enhanced the biodegradation of the aromatic compound of interest. Results also indicated JS6 has the ability to simultaneously degrade two aromatic compounds when the following mixtures were tested; toluene and benzene, toluene and chlorobenzene, and chlorobenzene and benzene. Enzyme induction experiments confirmed that the enzymes required for the initial breakdown of the aromatic compounds tested are inducible, not constitutive.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Microbiology.; Engineering, Civil.; Environmental Sciences.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Logan, Bruce E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleBiodegradation of aromatic compounds in the presence of secondary substratesen_US
dc.creatorVogel, Catherine, 1959-en_US
dc.contributor.authorVogel, Catherine, 1959-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractExperiments were conducted to examine the biodegradability of three aromatic compounds typically found at groundwater contamination sites; benzene, toluene, and chlorobenzene. A pure Pseudomonas species, JS6, was used in all batch experiments. JS6 was grown on benzene, chlorobenzene, toluene, yeast extract, or glucose as sole source of carbon and energy. These cultures were in turn used to test the biodegradability of the three aromatics of interest both in the presence and absence of the chemical used for acclimation. The results indicated that the presence of a non-aromatic substrate (yeast extract or glucose) enhanced the biodegradation of the aromatic compound of interest. Results also indicated JS6 has the ability to simultaneously degrade two aromatic compounds when the following mixtures were tested; toluene and benzene, toluene and chlorobenzene, and chlorobenzene and benzene. Enzyme induction experiments confirmed that the enzymes required for the initial breakdown of the aromatic compounds tested are inducible, not constitutive.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Microbiology.en_US
dc.subjectEngineering, Civil.en_US
dc.subjectEnvironmental Sciences.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLogan, Bruce E.en_US
dc.identifier.proquest1343835en_US
dc.identifier.bibrecord.b26882668en_US
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