Inactivation of bacteriophages MS-2 and PRD1 and poliovirus type 1 in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge

Persistent Link:
http://hdl.handle.net/10150/277884
Title:
Inactivation of bacteriophages MS-2 and PRD1 and poliovirus type 1 in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge
Author:
Straub, Timothy Mark, 1963-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Inactivation of bacteriophages MS-2 and PRD1, and poliovirus type 1 were measured in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge. Inactivation was determined at 15°C, 27°C, and 40°C with soils maintained at 30% moisture, and amended soils exposed to evaporation. No inactivation of PRD1 was observed within 30 days and 16 days at 15°C and 27°C respectively with little inactivation after 7 days at 40°C. Inactivation of MS-2 and poliovirus was approximately 2 fold greater at 27°C compared to 16°C. At 40°C neither virus was recovered 24 hours after sludge amendment. Evaporation to less than 5% soil moisture resulted in rapid loss of titer for all three viruses regardless of temperature. Survival of MS-2 and poliovirus 1 in sludge amended fields (15°C) was longer than comparable laboratory studies. None of the viruses were recovered in fields amended during the summer (33°C) after 7 days. A method was also developed to remove cell culture toxic components from these soils.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Agriculture, Food Science and Technology.; Biology, Microbiology.; Health Sciences, Public Health.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Gerba, Charles P.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleInactivation of bacteriophages MS-2 and PRD1 and poliovirus type 1 in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludgeen_US
dc.creatorStraub, Timothy Mark, 1963-en_US
dc.contributor.authorStraub, Timothy Mark, 1963-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractInactivation of bacteriophages MS-2 and PRD1, and poliovirus type 1 were measured in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge. Inactivation was determined at 15°C, 27°C, and 40°C with soils maintained at 30% moisture, and amended soils exposed to evaporation. No inactivation of PRD1 was observed within 30 days and 16 days at 15°C and 27°C respectively with little inactivation after 7 days at 40°C. Inactivation of MS-2 and poliovirus was approximately 2 fold greater at 27°C compared to 16°C. At 40°C neither virus was recovered 24 hours after sludge amendment. Evaporation to less than 5% soil moisture resulted in rapid loss of titer for all three viruses regardless of temperature. Survival of MS-2 and poliovirus 1 in sludge amended fields (15°C) was longer than comparable laboratory studies. None of the viruses were recovered in fields amended during the summer (33°C) after 7 days. A method was also developed to remove cell culture toxic components from these soils.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectAgriculture, Food Science and Technology.en_US
dc.subjectBiology, Microbiology.en_US
dc.subjectHealth Sciences, Public Health.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorGerba, Charles P.en_US
dc.identifier.proquest1343829en_US
dc.identifier.bibrecord.b26882607en_US
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