Loss of heterozygosity for loci on chromosome 6q in human malignant melanoma

Persistent Link:
http://hdl.handle.net/10150/277878
Title:
Loss of heterozygosity for loci on chromosome 6q in human malignant melanoma
Author:
Millikin, Dina Maria, 1953-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Restriction fragment length polymorphism (RFLP) analysis was used as a molecular genetic approach to examine loci on chromosome 6q for loss of constitutional heterozygosity (LOH). Five polymorphic DNA markers (that recognize RFLP's at specific chromosome 6q loci), and one polymorphic DNA marker for 6p were used to screen 20 autologous pairs of melanoma tumor DNA and normal blood DNA to determine the tumor and constitutional genotypes of each patient. LOH on chromosome 6q was identified at 21/53 informative loci (40%). Five patients had allele losses consistent with the loss of 6q (or of an entire copy of 6), and four other patients had terminal deletions of 6q. The chromosomal region bearing the highest frequency of 6q allele loss (60%) is defined by the marker loci c-MYB and ESR (q22-23 and q24-27). Comparison was then made with LOH observed at loci on four different chromosomes (1p, 11p, 16q and 17p) with results indicating a loss in 5% of cases. These results suggest that the loss of somatic sequences from chromosome 6q is a nonrandom and possibly biologically relevant event in human malignant melanoma.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Biology, Genetics.; Engineering, Biomedical.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Trent, Jeffrey M.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleLoss of heterozygosity for loci on chromosome 6q in human malignant melanomaen_US
dc.creatorMillikin, Dina Maria, 1953-en_US
dc.contributor.authorMillikin, Dina Maria, 1953-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractRestriction fragment length polymorphism (RFLP) analysis was used as a molecular genetic approach to examine loci on chromosome 6q for loss of constitutional heterozygosity (LOH). Five polymorphic DNA markers (that recognize RFLP's at specific chromosome 6q loci), and one polymorphic DNA marker for 6p were used to screen 20 autologous pairs of melanoma tumor DNA and normal blood DNA to determine the tumor and constitutional genotypes of each patient. LOH on chromosome 6q was identified at 21/53 informative loci (40%). Five patients had allele losses consistent with the loss of 6q (or of an entire copy of 6), and four other patients had terminal deletions of 6q. The chromosomal region bearing the highest frequency of 6q allele loss (60%) is defined by the marker loci c-MYB and ESR (q22-23 and q24-27). Comparison was then made with LOH observed at loci on four different chromosomes (1p, 11p, 16q and 17p) with results indicating a loss in 5% of cases. These results suggest that the loss of somatic sequences from chromosome 6q is a nonrandom and possibly biologically relevant event in human malignant melanoma.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectBiology, Genetics.en_US
dc.subjectEngineering, Biomedical.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorTrent, Jeffrey M.en_US
dc.identifier.proquest1343817en_US
dc.identifier.bibrecord.b26882474en_US
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