Sequence analysis of a follicle cell-specific gene from the mosquito, Aedes aegypti

Persistent Link:
http://hdl.handle.net/10150/277858
Title:
Sequence analysis of a follicle cell-specific gene from the mosquito, Aedes aegypti
Author:
Lin, Yonggu, 1963-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The follicle cell-specific (FCS) gene, a 3,023 bp gene specific to follicle cells of the mosquito, Aedes aegypti, was characterized at the nucleotide level. Genomic Southern blots demonstrated that there was only one copy of this gene in the A. aegypti genome. An ovary-specific cDNA library was constructed from female mosquitoes 24 hours post blood meal. Then a cDNA clone containing the complete coding region was identified, and its nucleotide sequence was determined. The deduced protein contained unusually high levels of alanine and proline. Search of a protein data base revealed that the FCS gene was similar to Drosophila vitelline membrane protein genes, with 47.5% similarity in nucleotide sequence and 46.7% similarity in amino acid sequence. The conserved hydrophobic regions from several vitelline membrane proteins were compared. Another cDNA clone, 1D, was isolated from the cDNA library screened with the FCS gene. These two mosquito genes shared a 60% similarity at the nucleic acid level and a 79.8% similarity at the amino acid level.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Genetics.; Biology, Entomology.; Biology, Genetics.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleSequence analysis of a follicle cell-specific gene from the mosquito, Aedes aegyptien_US
dc.creatorLin, Yonggu, 1963-en_US
dc.contributor.authorLin, Yonggu, 1963-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe follicle cell-specific (FCS) gene, a 3,023 bp gene specific to follicle cells of the mosquito, Aedes aegypti, was characterized at the nucleotide level. Genomic Southern blots demonstrated that there was only one copy of this gene in the A. aegypti genome. An ovary-specific cDNA library was constructed from female mosquitoes 24 hours post blood meal. Then a cDNA clone containing the complete coding region was identified, and its nucleotide sequence was determined. The deduced protein contained unusually high levels of alanine and proline. Search of a protein data base revealed that the FCS gene was similar to Drosophila vitelline membrane protein genes, with 47.5% similarity in nucleotide sequence and 46.7% similarity in amino acid sequence. The conserved hydrophobic regions from several vitelline membrane proteins were compared. Another cDNA clone, 1D, was isolated from the cDNA library screened with the FCS gene. These two mosquito genes shared a 60% similarity at the nucleic acid level and a 79.8% similarity at the amino acid level.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Genetics.en_US
dc.subjectBiology, Entomology.en_US
dc.subjectBiology, Genetics.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1343698en_US
dc.identifier.bibrecord.b26843948en_US
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