Characterization of polyclonal antibodies raised against DNA attachment proteins

Persistent Link:
http://hdl.handle.net/10150/277854
Title:
Characterization of polyclonal antibodies raised against DNA attachment proteins
Author:
Coeur, Vera Mae Fran, 1960-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The eukaryotic nucleus is thought to contain an internal protein scaffolding structure which is present in interphase and metaphase cells. We were interested in identifying some of the protein components of the scaffolding structure by isolating proteins which have DNA attached to them (DNA attachment proteins). DNA attachment proteins have been previously described by our group, and are referred to as proteins 1, 2, 3, and 4, respectively. Their apparent molecular weights (Mr) and isoelectric values (pI) are 70,000, 4.3; 70,000, 5.3; 58,000, 5.3; and 57,000, 4.8, respectively. My project was to produce and characterize polyclonal antibodies made against these four proteins. The DNA attachment proteins were injected into rabbits. The serum was affinity purified using Protein-A sepharose chromatography. Antibody preparations 1, 2, and 4 recognized the specific DNA attachment proteins and did not cross react with the others suggesting that the polyclonal sera recognized distinct and separate epitopes.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Molecular.; Biology, Microbiology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Cress, Anne E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleCharacterization of polyclonal antibodies raised against DNA attachment proteinsen_US
dc.creatorCoeur, Vera Mae Fran, 1960-en_US
dc.contributor.authorCoeur, Vera Mae Fran, 1960-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe eukaryotic nucleus is thought to contain an internal protein scaffolding structure which is present in interphase and metaphase cells. We were interested in identifying some of the protein components of the scaffolding structure by isolating proteins which have DNA attached to them (DNA attachment proteins). DNA attachment proteins have been previously described by our group, and are referred to as proteins 1, 2, 3, and 4, respectively. Their apparent molecular weights (Mr) and isoelectric values (pI) are 70,000, 4.3; 70,000, 5.3; 58,000, 5.3; and 57,000, 4.8, respectively. My project was to produce and characterize polyclonal antibodies made against these four proteins. The DNA attachment proteins were injected into rabbits. The serum was affinity purified using Protein-A sepharose chromatography. Antibody preparations 1, 2, and 4 recognized the specific DNA attachment proteins and did not cross react with the others suggesting that the polyclonal sera recognized distinct and separate epitopes.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Molecular.en_US
dc.subjectBiology, Microbiology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorCress, Anne E.en_US
dc.identifier.proquest1343691en_US
dc.identifier.bibrecord.b26843870en_US
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