Pharmacological characterization of peripheral-type benzodiazepine receptor found in mitochondrial and microsomal fractions of the rat liver, heart and kidney

Persistent Link:
http://hdl.handle.net/10150/277848
Title:
Pharmacological characterization of peripheral-type benzodiazepine receptor found in mitochondrial and microsomal fractions of the rat liver, heart and kidney
Author:
Li, Hong Bing, 1966-
Issue Date:
1991
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In the present study, we have demonstrated that PBR sites are present in the microsomal fraction from rat liver, heart and kidney, which we have named the non-mitochondrial PBR. The non-mitochondrial PBR was pharmacologically characterized by porphyrin competition experiments. The ability of porphyrins to differentially compete for specific (3H) Ro5-4864 or (3H) PK11195 binding demonstrated that the mitochondrial and non-mitochondrial PBR have different affinities for selected porphyrin compounds. These results suggest that the mitochondrial and non-mitochondrial PBR have a different binding pharmacology and support the existence of a non-mitochondrial PBR site. On the other hand, porphyrins show different potencies in competing for specific (3H) Ro5-4864 and (3H) PK11195 binding in the same fraction. In addition, the results of saturation experiments show that there are more PK11195 binding sites than Ro5-4864 binding sites in all fractions examined. This observation suggests that the sites labeled by (3H) Ro5-4864 and (3H) PK11195 are not identical.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Health Sciences, Pharmacology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Laird, Hugh E., II

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titlePharmacological characterization of peripheral-type benzodiazepine receptor found in mitochondrial and microsomal fractions of the rat liver, heart and kidneyen_US
dc.creatorLi, Hong Bing, 1966-en_US
dc.contributor.authorLi, Hong Bing, 1966-en_US
dc.date.issued1991en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn the present study, we have demonstrated that PBR sites are present in the microsomal fraction from rat liver, heart and kidney, which we have named the non-mitochondrial PBR. The non-mitochondrial PBR was pharmacologically characterized by porphyrin competition experiments. The ability of porphyrins to differentially compete for specific (3H) Ro5-4864 or (3H) PK11195 binding demonstrated that the mitochondrial and non-mitochondrial PBR have different affinities for selected porphyrin compounds. These results suggest that the mitochondrial and non-mitochondrial PBR have a different binding pharmacology and support the existence of a non-mitochondrial PBR site. On the other hand, porphyrins show different potencies in competing for specific (3H) Ro5-4864 and (3H) PK11195 binding in the same fraction. In addition, the results of saturation experiments show that there are more PK11195 binding sites than Ro5-4864 binding sites in all fractions examined. This observation suggests that the sites labeled by (3H) Ro5-4864 and (3H) PK11195 are not identical.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectHealth Sciences, Pharmacology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLaird, Hugh E., IIen_US
dc.identifier.proquest1343613en_US
dc.identifier.bibrecord.b26807099en_US
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