Evaluation of immunochemical assays for on-site determination of aflatoxin in cottonseed

Persistent Link:
http://hdl.handle.net/10150/277801
Title:
Evaluation of immunochemical assays for on-site determination of aflatoxin in cottonseed
Author:
Njapau, Henry, 1956-
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The performance of four immunochemical test kits in detecting aflatoxin contamination in cottonseed under non-laboratory conditions was evaluated. Naturally contaminated cottonseed was analyzed at cotton gins, ammoniation plants, cotton oil processing plants, a dairy farm and at the University of Arizona. Evaluation was based on the ability to determine aflatoxin concentrations as more or less than 20 ppb when analyzed on-site and in the laboratory using a common extract. Comparison of results from both locations showed excellent agreement (Afla-20 Cup 86 ± 6%, (n = 188); Agri-Screen I, 75 ± 9%, (n = 56); Agri-Screen II, 93 ± 5%, (n = 106); Agri-Chek, 93 ± 4%, (n = 156) and Aflatest-10, 90 ± 4%, (n = 178)). Overall agreement between on-site and laboratory results was 88%. Comparison of kit results to those of TLC demonstrated a low potential for obtaining false negative values. From these results the kits can reliably be used to screen cottonseed for aflatoxin contamination under non-laboratory conditions.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Agriculture, Food Science and Technology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Park, Douglas L.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleEvaluation of immunochemical assays for on-site determination of aflatoxin in cottonseeden_US
dc.creatorNjapau, Henry, 1956-en_US
dc.contributor.authorNjapau, Henry, 1956-en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe performance of four immunochemical test kits in detecting aflatoxin contamination in cottonseed under non-laboratory conditions was evaluated. Naturally contaminated cottonseed was analyzed at cotton gins, ammoniation plants, cotton oil processing plants, a dairy farm and at the University of Arizona. Evaluation was based on the ability to determine aflatoxin concentrations as more or less than 20 ppb when analyzed on-site and in the laboratory using a common extract. Comparison of results from both locations showed excellent agreement (Afla-20 Cup 86 ± 6%, (n = 188); Agri-Screen I, 75 ± 9%, (n = 56); Agri-Screen II, 93 ± 5%, (n = 106); Agri-Chek, 93 ± 4%, (n = 156) and Aflatest-10, 90 ± 4%, (n = 178)). Overall agreement between on-site and laboratory results was 88%. Comparison of kit results to those of TLC demonstrated a low potential for obtaining false negative values. From these results the kits can reliably be used to screen cottonseed for aflatoxin contamination under non-laboratory conditions.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectAgriculture, Food Science and Technology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorPark, Douglas L.en_US
dc.identifier.proquest1342980en_US
dc.identifier.bibrecord.b26622737en_US
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