Effect of Corynebacterium pseudotuberculosis phospholipase D on ovine neutrophil function

Persistent Link:
http://hdl.handle.net/10150/277329
Title:
Effect of Corynebacterium pseudotuberculosis phospholipase D on ovine neutrophil function
Author:
Yozwiak, Michael Leo, 1963-
Issue Date:
1990
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Corynebacterium pseudotuberculosis causes caseous lymphadenitis of sheep and goats and produces a phospholipase D (PLD) exotoxin which is putatively important in pathogenesis. Viability and function of ovine polymorphonuclear leukocytes (PMN) treated with crude and purified forms of PLD were determined by various assays. PMN viability by dye exclusion showed the PLD-treatment had a significant effect only after 24 hour incubation. Scanning electron microscopy of PLD-treated ovine erythrocytes revealed membrane alterations, but no such alterations were seen in PLD-treated PMN. Transmission electron microscopy revealed significantly fewer granules in PMN treated with PLD, although other facets of phagocytic function appeared to be normal, including phagosome-lysosome fusion. PLD-treated PMN were significantly reduced in their ability to internalize Corynebacterium pseudotuberculosis and to attach to or phagocytose Staphylococcus epidermidis. Purified PLD activated normal sheep serum, producing chemotactic factors. PLD treatment of PMN significantly reduced the ability of these cells to migrate toward activated sheep serum.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Biology, Microbiology.; Biology, Veterinary Science.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College
Degree Grantor:
University of Arizona
Advisor:
Songer, J. Glenn

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleEffect of Corynebacterium pseudotuberculosis phospholipase D on ovine neutrophil functionen_US
dc.creatorYozwiak, Michael Leo, 1963-en_US
dc.contributor.authorYozwiak, Michael Leo, 1963-en_US
dc.date.issued1990en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractCorynebacterium pseudotuberculosis causes caseous lymphadenitis of sheep and goats and produces a phospholipase D (PLD) exotoxin which is putatively important in pathogenesis. Viability and function of ovine polymorphonuclear leukocytes (PMN) treated with crude and purified forms of PLD were determined by various assays. PMN viability by dye exclusion showed the PLD-treatment had a significant effect only after 24 hour incubation. Scanning electron microscopy of PLD-treated ovine erythrocytes revealed membrane alterations, but no such alterations were seen in PLD-treated PMN. Transmission electron microscopy revealed significantly fewer granules in PMN treated with PLD, although other facets of phagocytic function appeared to be normal, including phagosome-lysosome fusion. PLD-treated PMN were significantly reduced in their ability to internalize Corynebacterium pseudotuberculosis and to attach to or phagocytose Staphylococcus epidermidis. Purified PLD activated normal sheep serum, producing chemotactic factors. PLD treatment of PMN significantly reduced the ability of these cells to migrate toward activated sheep serum.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBiology, Microbiology.en_US
dc.subjectBiology, Veterinary Science.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorSonger, J. Glennen_US
dc.identifier.proquest1341242en_US
dc.identifier.bibrecord.b26330970en_US
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