In vivo protein turnover and the influence of ecdysteroids in flight muscle of the tobacco hornworm, Manduca sexta

Persistent Link:
http://hdl.handle.net/10150/277242
Title:
In vivo protein turnover and the influence of ecdysteroids in flight muscle of the tobacco hornworm, Manduca sexta
Author:
Wu, Min, 1958-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In vivo protein turnover was measured during the last 4 days of flight muscle development in tobacco hornworm pupa. Linear synthesis rates were measured up to 2 hours after injection of 30 μmol (3H) phenylalanine. Since the results with this technique did not differ from another established method, the large bolus injection of phenylalanine did not affect protein synthesis. The former method is advantageous because only a single time point is required. Flight muscle growth and protein synthesis decreased in parallel between 100 and 24 hours. During this time free phenylalanine turnover decreased, and the total pool diminished, indicating that this pool could be a major sink for muscle protein synthesis. Proteolysis was rapid even in the growing muscle. 20-Hydroxyecdysone increased muscle growth at certain times by inhibiting proteolysis. Protein synthesis either decreased or was unchanged after injection of the hormone. Therefore ecdysteroids may play a role in controlling growth of the dorsolongitudinal flight muscle during adult development, especially by retarding proteolysis.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Manduca.; Tobacco hornworm.; Ecdysteroids.; Muscle proteins.; Insects -- Flight.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Nutritional Sciences
Degree Grantor:
University of Arizona
Advisor:
Tischler, Marc E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleIn vivo protein turnover and the influence of ecdysteroids in flight muscle of the tobacco hornworm, Manduca sextaen_US
dc.creatorWu, Min, 1958-en_US
dc.contributor.authorWu, Min, 1958-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn vivo protein turnover was measured during the last 4 days of flight muscle development in tobacco hornworm pupa. Linear synthesis rates were measured up to 2 hours after injection of 30 μmol (3H) phenylalanine. Since the results with this technique did not differ from another established method, the large bolus injection of phenylalanine did not affect protein synthesis. The former method is advantageous because only a single time point is required. Flight muscle growth and protein synthesis decreased in parallel between 100 and 24 hours. During this time free phenylalanine turnover decreased, and the total pool diminished, indicating that this pool could be a major sink for muscle protein synthesis. Proteolysis was rapid even in the growing muscle. 20-Hydroxyecdysone increased muscle growth at certain times by inhibiting proteolysis. Protein synthesis either decreased or was unchanged after injection of the hormone. Therefore ecdysteroids may play a role in controlling growth of the dorsolongitudinal flight muscle during adult development, especially by retarding proteolysis.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectManduca.en_US
dc.subjectTobacco hornworm.en_US
dc.subjectEcdysteroids.en_US
dc.subjectMuscle proteins.en_US
dc.subjectInsects -- Flight.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineNutritional Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorTischler, Marc E.en_US
dc.identifier.proquest1339278en_US
dc.identifier.oclc24324750en_US
dc.identifier.bibrecord.b17852341en_US
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