Confirmation of urinary benzodiazepines by gas chromatography/mass spectrometry

Persistent Link:
http://hdl.handle.net/10150/277228
Title:
Confirmation of urinary benzodiazepines by gas chromatography/mass spectrometry
Author:
West, Robert E., 1952-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
A new method is described for the quantitative analysis of urinary benzodiazepines by gas chromatography/mass spectrometry. Development work was aimed at satisfying federal requirements for methods used in forensic urine drug testing which have become the standard in the laboratory industry. Trimethylsilyl (TMS), tert-butyl-dimethylsilyl (TBDMS) and benzophenone derivatives were tested in the development of the new assay. TBDMS derivatives were found to be the most suitable for the analysis of six common benzodiazepine metabolites. Precision for all metabolites tested, as measured by the within run coefficient of variation, was less than 7% at 100 ng/ml (n = 15). Assay sensitivity varied with the specific analyte in the range of 5 to 10 ng/ml. Validation of the procedure included the reanalysis of benzodiazepine positive urine specimens obtained from a forensic drug testing laboratory and comparison of the results from the independent assays. These specimens were tested first by radioimmunoassay using a 100 ng/ml cutoff and then confirmed by GC/MS. Sensitivity was sufficient to confirm the presence of benzodiazepine metabolites in all specimens tested.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Benzodiazepines.; Drug testing.; Urine -- Analysis.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Pharmacology and Toxicology
Degree Grantor:
University of Arizona
Advisor:
Carter, Dean

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleConfirmation of urinary benzodiazepines by gas chromatography/mass spectrometryen_US
dc.creatorWest, Robert E., 1952-en_US
dc.contributor.authorWest, Robert E., 1952-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractA new method is described for the quantitative analysis of urinary benzodiazepines by gas chromatography/mass spectrometry. Development work was aimed at satisfying federal requirements for methods used in forensic urine drug testing which have become the standard in the laboratory industry. Trimethylsilyl (TMS), tert-butyl-dimethylsilyl (TBDMS) and benzophenone derivatives were tested in the development of the new assay. TBDMS derivatives were found to be the most suitable for the analysis of six common benzodiazepine metabolites. Precision for all metabolites tested, as measured by the within run coefficient of variation, was less than 7% at 100 ng/ml (n = 15). Assay sensitivity varied with the specific analyte in the range of 5 to 10 ng/ml. Validation of the procedure included the reanalysis of benzodiazepine positive urine specimens obtained from a forensic drug testing laboratory and comparison of the results from the independent assays. These specimens were tested first by radioimmunoassay using a 100 ng/ml cutoff and then confirmed by GC/MS. Sensitivity was sufficient to confirm the presence of benzodiazepine metabolites in all specimens tested.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBenzodiazepines.en_US
dc.subjectDrug testing.en_US
dc.subjectUrine -- Analysis.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePharmacology and Toxicologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorCarter, Deanen_US
dc.identifier.proquest1339237en_US
dc.identifier.oclc24324693en_US
dc.identifier.bibrecord.b18424338en_US
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